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首页> 外文期刊>Journal of genetics >Characterization and tissue distribution of Lhx9 and Lhx9?± in Chinese giant salamander Andrias davidianus
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Characterization and tissue distribution of Lhx9 and Lhx9?± in Chinese giant salamander Andrias davidianus

机译：中国巨型salAndrias davidianus中Lhx9和Lhx9？±的特征和组织分布

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摘要

Lhx9 is an LIM (named for the first three proteins in which the domain was found, Lin-11, Isl1 and Mec-3) homeodomain protein involved in development and differentiation of the gonad. In this study, we isolated the full-length Lhx9 and Lhx9?± from Andrias davidianus, detected the tissue distribution and analysed the methylation of the promoters. We identified Lhx9 of 1411 bp and Lhx9?± of 1153-bp length, differing in the 3'-flanking region, encoding 399 and 330 amino acids, respectively. The Lhx9 gene was detected primarily in liver, ovary and heart with moderate expression in brain, pituitary, intestine and spleen, and low expression in the remaining examined tissues, while Lhx9 ?± expression was high in heart, pituitary and liver, and low in spleen and stomach. Significantly higher Lhx9 expression was observed in ovary than in testis, with no differences in Lhx9?± expression between testis and ovary observed. Bisulphite sequencing revealed significantly higher methylation in testis compared to ovary. The methylation level of CpG sites a€“733, a€“673, a€“615 and a€“594 exhibited significantly higher levels in testis than in ovary, which was negatively correlated with Lhx9 expression. The methylation and expression patterns suggested that promoter methylation suppressed expression of Lhx9 in A. davidianus.
机译：Lhx9是一种LIM（以发现该域的前三个蛋白Lin-11，Isl1和Mec-3命名）同源域蛋白，参与性腺的发育和分化。在这项研究中，我们从Andrias davidianus分离了全长的Lhx9和Lhx9α±，检测了组织分布并分析了启动子的甲基化。我们鉴定出长度为1411 bp的Lhx9和长度为1153 bp的Lhx9α±，在3'侧翼区域不同，分别编码399和330个氨基酸。 Lhx9基因主要在肝，卵巢和心脏中检测到，在脑，垂体，肠和脾中表达适中，在其余受检组织中低表达，而Lhx9α±在心脏，垂体和肝脏中高表达，而在肝，卵巢和心脏中低表达。脾胃虚弱。在卵巢中观察到的Lhx9表达明显高于睾丸，在睾丸和卵巢之间观察到的Lhx9α±表达没有差异。亚硫酸氢盐测序显示，与卵巢相比，睾丸中的甲基化明显更高。 CpG位点733、673、615和594的甲基化水平显着高于卵巢，这与Lhx9表达负相关。甲基化和表达模式表明启动子甲基化抑制了拟南芥中Lhx9的表达。

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