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首页> 外文期刊>Journal of Extracellular Vesicles >Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids
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Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids

机译：快速侧流免疫测定试验的开发，用于检测先前从细胞培养基和体液中富集的外来体

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Exosomes are cell-secreted nanovesicles (40–200 nm) that represent a rich source of novel biomarkers in the diagnosis and prognosis of certain diseases. Despite the increasingly recognized relevance of these vesicles as biomarkers, their detection has been limited due in part to current technical challenges in the rapid isolation and analysis of exosomes. The complexity of the development of analytical platforms relies on the heterogeneous composition of the exosome membrane. One of the most attractive tests is the inmunochromatographic strips, which allow rapid detection by unskilled operators. We have successfully developed a novel lateral flow immunoassay (LFIA) for the detection of exosomes based on the use of tetraspanins as targets. We have applied this platform for the detection of exosomes purified from different sources: cell culture supernatants, human plasma and urine. As proof of concept, we explored the analytical potential of this LFIA platform to accurately quantify exosomes purified from a...
机译：外泌体是细胞分泌的纳米囊泡（40-200 nm），在某些疾病的诊断和预后中代表着新颖的生物标志物的丰富来源。尽管人们日益认识到这些囊泡作为生物标志物的相关性，但其检测一直受到限制，部分原因是在快速分离和分析外泌体方面存在当前的技术挑战。分析平台开发的复杂性取决于外泌体膜的异质组成。免疫色谱条是最有吸引力的测试之一，可让非熟练操作人员快速检测。我们已经成功地开发了一种新型的侧流免疫测定法（LFIA），用于以四跨膜蛋白为靶标检测外泌体。我们已将该平台用于检测从不同来源纯化的外泌体：细胞培养上清液，人血浆和尿液。作为概念验证，我们探索了该LFIA平台的分析潜力，可以准确地定量从ALPHA纯化的外泌体。

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