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首页> 外文期刊>Journal of Extracellular Vesicles >High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources
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High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources

机译：来自不同细胞来源的外泌体和微泡的高分辨率蛋白质组学和脂质组学分析

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摘要

Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were more likely different from their cells of origin than were the protein patterns of MVs; (b) The proteomes of U87 and Huh7 exosomes were similar to each other but different from the proteomes of MSC exosomes, whereas the lipidomes of Huh7 and MSC exosomes were similar to each other but different from the lipidomes of U87 exosomes; (c) exosomes exhibited proteins of extracellular matrix, heparin-binding, receptors, immune response and cell adhesion functions, whereas MVs were enriched in endoplasmic reticulum, proteasome and mitochondrial proteins. Exosomes and MVs also differed in their types of lipid contents. Enrichment in glycolipids and free fatty acids characterized exosomes, whereas enrichment in ceramides and sphingomyelins characterized MVs. Furthermore, Huh7 and MSC exosomes were specifically enriched in cardiolipins; U87 exosomes were enriched in sphingomyelins. This study comprehensively analyses the protein and lipid composition of exosomes, MVs and source cells in 3 different cell types.
机译：研究了包括外泌体和微囊泡（MVs）在内的细胞外囊泡（EVs）在诊断，治疗和药物递送中的用途。但是，关于电动汽车及其源细胞的蛋白质和脂质组成之间的关系知之甚少。在这里，我们报告了通过差异超速离心从3种不同的细胞类型衍生而来的外泌体和MV的高分辨率脂质组学和蛋白质组学分析：U87胶质母细胞瘤细胞，Huh7肝细胞癌细胞和人骨髓来源的间充质干细胞（MSCs）。我们在屏幕上鉴定了3,532种蛋白质和1,961种脂质。外泌体在几个不同方面与MV不同：（a）与MVs的蛋白质模式相比，exosome的蛋白质模式与原代细胞的差异更大; （b）U87和Huh7外泌体的蛋白质组彼此相似，但与MSC外泌体的蛋白质组不同，而Huh7和MSC外泌体的脂质组彼此相似，但不同于U87外泌体的脂质组。 （c）外泌体表现出细胞外基质，肝素结合，受体，免疫反应和细胞粘附功能的蛋白质，而MV富含内质网，蛋白酶体和线粒体蛋白质。外来体和MV的脂质含量也有所不同。糖脂和游离脂肪酸的富集表征外来体，而神经酰胺和鞘磷脂的富集表征MV。此外，Huh7和MSC外泌体特别富含心磷脂。 U87外泌体富含鞘磷脂。这项研究全面分析了三种不同细胞类型中外泌体，MV和源细胞的蛋白质和脂质组成。

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