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首页> 外文期刊>Journal of genetics >Ubiquitous distribution of fluorescent protein in muscles of four species and two subspecies of eel (genus Anguilla)
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Ubiquitous distribution of fluorescent protein in muscles of four species and two subspecies of eel (genus Anguilla)

机译：荧光蛋白在鳗鱼四个属和两个亚种的肌肉中的普遍分布

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In this study, the localization of fluorescent protein (FP) was characterized in the muscles of four species and two subspecies of eels Anguilla anguilla, A. australis, A. bicolor bicolor (b.), A. bicolor pacifica (p.) and A. mossambica in addition tothe previously reported A. japonica. The open reading frame of each eel FP was 417 bp encoding 139 amino acid residues. The deduced amino acid sequences among the four species and two subspecies exhibited 91.4a€“100% identity, and belonged to the fatty-acid-binding protein (FABP) family. The gene structure of eel FPs in A. japonica, A. anguilla, A. australis, A. bicolor b., A. bicolor p. and A. mossambica have four exons and three introns, and were common to that of FABP family. The apo eel FPs expressed by Escherichia coli with recombinant eel FP genes were analysed for the fluorescent properties in the presenceof bilirubin. The excitation and emission spectra of holo eel FPs had the maximum wavelengths of 490a€“496 and 527a€“530 nm, respectively. The holo eel FPs indicated that the fluorescent intensities were stronger in A. japonica and A. bicolor than in A. mossambica, A. australis and A. anguilla. The comparison of amino acid sequences revealed two common substitutions in A.mossambica, A. australis and A. anguilla with weak fluorescent intensity.
机译：在这项研究中，荧光蛋白（FP）的定位在鳗species，鳗A，双色双色（b。），双色双歧杆菌（p。）和四色鳗两个物种中的特征。除先前报道的粳稻以外，还有莫桑比克蘑菇。每个鳗鱼FP的开放阅读框为417bp，编码139个氨基酸残基。推导的四个物种和两个亚种之间的氨基酸序列具有91.4a-100％的同一性，属于脂肪酸结合蛋白（FABP）家族。粳稻，安圭拉瓜，南极虾，双色鲍鱼，双色鲍鱼的鳗鱼FPs的基因结构。 A. mossambica和A. mossambica具有4个外显子和3个内含子，与FABP家族相同。在存在胆红素的情况下，分析了大肠杆菌表达的带有重组鳗FP基因的载脂鳗FP的荧光特性。全鳗鱼FPs的激发和发射光谱的最大波长分别为490a·496和527a·530nm。全鳗鱼FPs表明荧光强度在粳稻和双色青霉中比在莫桑比克青霉，澳大利亚青霉和安圭青霉中强。氨基酸序列的比较揭示了在莫桑比克曲霉中两个常见的取代，即澳大利亚曲霉和安圭曲霉，其荧光强度较弱。

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