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首页> 外文期刊>Journal of genetics >Ubiquitous distribution of fluorescent protein in muscles of four species and two subspecies of eel (genus Anguilla)
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Ubiquitous distribution of fluorescent protein in muscles of four species and two subspecies of eel (genus Anguilla)

机译：四种物种肌肉中荧光蛋白的无处不在的分布和鳗鱼（Genus Anguilla）

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摘要

In this study, the localization of fluorescent protein (FP) was characterized in the muscles of four species and two subspecies of eels Anguilla anguilla, A. australis, A. bicolor bicolor (b.), A. bicolor pacifica (p.) and A. mossambica in addition to the previously reported A. japonica. The open reading frame of each eel FP was 417 bp encoding 139 amino acid residues. The deduced amino acid sequences among the four species and two subspecies exhibited 91.4-100% identity, and belonged to the fatty-acid-binding protein (FABP) family. The gene structure of eel FPs in A. japonica, A. anguilla, A. australis, A. bicolor b., A. bicolor p. and A. mossambica have four exons and three introns, and were common to that of FABP family. The apo eel FPs expressed by Escherichia coli with recombinant eel FP genes were analysed for the fluorescent properties in the presence of bilirubin. The excitation and emission spectra of holo eel FPs had the maximum wavelengths of 490-496 and 527-530 nm, respectively. The holo eel FPs indicated that the fluorescent intensities were stronger in A. japonica and A. bicolor than in A. mossambica, A. australis and A. anguilla. The comparison of amino acid sequences revealed two common substitutions in A. mossambica, A. australis and A. anguilla with weak fluorescent intensity.
机译：在这项研究中，荧光蛋白（FP）的定位的特征在于四种物种和两种菌株的鳗鱼，A.澳大利多，A.Bicolor双色（B.），A.Bicolor Pacifica（p。）和A. Mossambica除了先前报道的A. japonica之外。每个鳗鱼FP的开放阅读框为417bp编码139个氨基酸残基。四种物种和两个亚种中所推出的氨基酸序列表现出91.4-100％的同一性，属于脂肪酸结合蛋白（FABP）家族。鳗鱼FPS的基因结构在A. japonica，A. Anguilla，A. Australis，A. Bicolor B.，A. Bicolor P. A.Mossambica有四个外显子和三个内含子，并且对Fabp家族的共同点是共同的。在胆红素存在下分析具有重组鳗鱼FP基因的大肠杆菌Coli表达的APO鳗鱼FPS。 Holo Eel FP的激发和发射光谱分别具有490-496和527-530nm的最大波长。 Holo EEL FPS表明，荧光强度在A.粳稻和A.双子比A. Mossambica，A. Australis和A. Anguilla中较强。氨基酸序列的比较揭示了A.Mossambica，A. Australis和A. Anguilla的两种常见取代。

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