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首页> 外文期刊>Journal of genetics >De novo sequencing of the Antarctic krill (Euphausia superba) transcriptome to identify functional genes and molecular markers
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De novo sequencing of the Antarctic krill (Euphausia superba) transcriptome to identify functional genes and molecular markers

机译：从头开始对南极磷虾（Euphausia superba）转录组进行测序，以鉴定功能基因和分子标记

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To provide massive genetic resources for the Antarctic krill (Euphausia superba), we sequenced and analysed thetranscriptome by using high-throughput Illumina paired-end sequencing technology. A total of 77.1 million clean reads representing a??11.0Gb data were generated. The average length of these reads was 142 bp. De novo assembly yielded 125,211 transcripts with a N50of 690 bp. Further analysis produced 106,250 unigenes, of which 31,683 were annotated based on protein homology searches againstprotein databases. Gene ontology analysis showed that ion binding, organic substance, metabolic process, and cell part were the mostabundantly used terms in molecular function, biological process and cellular component categories, respectively. In addition, 3067 unigenes were mapped onto 311 signal pathways by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Finally, 15,224 simple sequence repeats were identified from 13,535 transcripts, and 103,593 single-nucleotide polymorphisms werefound from 21.6% of total transcripts. These genetic resources obtained in this study forms a good foundation for investigating genefunction, and evaluating population genetic diversity for this important Southern Ocean fisheries resource, E. superba.
机译：为了为南极磷虾（Euphausia superba）提供大量的遗传资源，我们使用高通量的Illumina配对末端测序技术对转录组进行了测序和分析。总共产生了7710万个干净读取，代表?? 11.0Gb数据。这些读段的平均长度为142 bp。从头组装产生125211个转录物，其N50为690bp。进一步分析产生了106,250个单基因，其中31,683个是基于对蛋白质数据库的蛋白质同源性搜索标注的。基因本体分析表明，离子结合，有机物，代谢过程和细胞部分分别是分子功能，生物过程和细胞成分类别中使用最多的术语。此外，通过《京都基因与基因组百科全书》（KEGG）途径分析，将3067个单基因定位到311条信号途径上。最后，从13,535个转录物中鉴定出15,224个简单序列重复，从总转录物中的21.6％发现103,593个单核苷酸多态性。在这项研究中获得的这些遗传资源为调查基因功能和评估这一重要的南方海洋渔业资源大肠埃希氏菌的种群遗传多样性奠定了良好的基础。

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