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首页> 外文期刊>Journal of genetics >Mutations in the e?‘? gene region of hepatitis B virus genotype e??· in Turkish patients
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Mutations in the e?‘? gene region of hepatitis B virus genotype e??· in Turkish patients

机译：e中的突变？’？土耳其患者乙型肝炎病毒基因型e ??·的基因区域

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The e?‘? gene region of the hepatitis B virus (HBV) is responsible for the expression of surface antigens and includes the a€?aa€?-determinant region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage, permitting the mutant virus to escape from the immune system. The aim of this study was to search for mutations of the e?‘? gene region in different patient groups infected with genotype e??· variants of HBV, and to analyse the biological significance of these mutations. Moreover, we investigated e?‘? gene mutation inductance among family members. Forty HBV-DNA-positive patients were determined among 132 hepatitis B surface antigen (HbsAg) carriers by the first stage of seminested PCR. Genotypes and subtypes were established by sequencing of the amplified S gene regions. Variants were compared with original sequences of these serotypes, and mutations were identified. All variants were designated as genotype e??· and subtype ayw3. Ten kinds of point mutations were identified within the e?‘? region. The highest rates of mutation were found in chronic hepatitis patients and their family members. The amino acid mutations 125 (M a?’ T) and 127 (T a?’ P) were found on the first loop of a€?aa€?-determinant. The other consequence was mutation inductance in a family member. We found some mutations in the S gene region known to be stable and observed that some of these mutations affected e?‘? gene expression.
机译：e？’？乙型肝炎病毒（HBV）的基因区域负责表面抗原的表达，并包括一个决定簇区域。因此，该区域的突变将为HBV变异提供独特的生存优势，从而使突变病毒能够逃离免疫系统。这项研究的目的是寻找e？’？的突变。将不同患者组中的基因区域感染了HBV基因型e ??·变异，并分析了这些突变的生物学意义。此外，我们调查了e？’？家庭成员之间的基因突变感应。通过第一阶段的半巢式PCR，确定了132例乙型肝炎表面抗原（HbsAg）携带者中的40例HBV-DNA阳性患者。通过对扩增的S基因区域进行测序来确定基因型和亚型。将变体与这些血清型的原始序列进行比较，并鉴定出突变。所有的变体都被指定为基因型e ??·和亚型ayw3。在e？’？内识别出十种点突变。地区。在慢性肝炎患者及其家人中发现突变率最高。在a决定氨基酸的第一个环上发现了氨基酸突变125（M a？'T）和127（T a？'P）。另一个结果是家庭成员中的突变电感。我们在已知的S基因区域发现了一些稳定的突变，并观察到其中一些突变影响了e？’？基因表达。

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