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首页> 外文期刊>Journal of Extracellular Vesicles >Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting
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Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting

机译：GPI锚定的抗EGFR纳米抗体在细胞外囊泡上的展示促进肿瘤细胞靶向

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BackgroundExtracellular vesicles (EVs) are attractive candidate drug delivery systems due to their ability to functionally transport biological cargo to recipient cells. However, the apparent lack of target cell specificity of exogenously administered EVs limits their therapeutic applicability. In this study, we propose a novel method to equip EVs with targeting properties, in order to improve their interaction with tumour cells.MethodsEV producing cells were transfected with vectors encoding for anti-epidermal growth factor receptor (EGFR) nanobodies, which served as targeting ligands for tumour cells, fused to glycosylphosphatidylinositol (GPI) anchor signal peptides derived from decay-accelerating factor (DAF). EVs were isolated using ultrafiltration/size-exclusion liquid chromatography and characterized using western blotting, Nanoparticle Tracking Analysis, and electron microscopy. EV–tumour cell interactions were analyzed under static conditions using flow cytometry and under flow conditions using a...
机译：背景技术由于细胞外囊泡（EVs）将生物货物功能性转运至受体细胞的能力，因此它们是有吸引力的候选药物递送系统。然而，外源施用的电动汽车明显缺乏靶细胞特异性限制了它们的治疗适用性。在这项研究中，我们提出了一种新颖的方法来为EV配备靶向特性，以改善它们与肿瘤细胞的相互作用。方法用编码抗表皮生长因子受体（EGFR）纳米抗体的载体转染EV生产细胞肿瘤细胞配体，与衍生自衰变促进因子（DAF）的糖基磷脂酰肌醇（GPI）锚定信号肽融合。使用超滤/尺寸排阻液相色谱法分离电动汽车，并使用蛋白质印迹，纳米颗粒跟踪分析和电子显微镜对电动汽车进行表征。 EV-肿瘤细胞相互作用在静态条件下使用流式细胞仪进行了分析，在流动条件下使用了流式细胞仪进行了分析。

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