...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Mapping of Id locus for dermal shank melanin in a Chinese indigenous chicken breed
【24h】

Mapping of Id locus for dermal shank melanin in a Chinese indigenous chicken breed

机译：中国本地鸡品种真皮小腿黑色素的Id位点定位

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The dermal shank pigmentation, one of the defining traits of chicken breeds, is caused by an abnormal deposition of melanin in the dermis of the shank. The abnormal deposition is controlled by the sex-linked inhibitor of dermal melanin (Id). In this study, we aim to locate the gene responsible for the dermal shank pigmentation in chickens by an association analysis and a differential expression analysis. Based on our results, 72 single-nucleotide polymorphisms (SNPs) located in Z chromosome (chrZ): 71–73 Mb (galGal3) were selected to further explore their relationships with the dermal shank pigmentation in pure lines of 96 Gushi hens and 96 Gushi hens with a yellow shank skin colour. The results of the association analysis showed that the SNPs located in chrZ: 72.58–72.99 Mb (galGal3) (chrZ: 79.02–79.44 Mb (galGal4)) are significantly associated with the dermal shank pigmentation. Based on the results of our previous studies and the present association analysis, the zinc-finger protein 608 (ZNF608), GRAM domain containing 3 (GRAMD3), aldehyde dehydrogenase 7 family member A1 (ALDH7A1), fem-1 homologue C (FEM1C), beta-1,4-galactosyltransferase 1 (B4GALT1) and versican (VCAN) genes were selected for the differential expression analysis. The gene expression profiles showed that the expression ofGRAMD3gene in the dermis tissues of the shank was significantly (P = 0.010738 0.05) higher in 350-day-old Gushi chickens characterized by the dermal shank pigmentation than in one-day-old Gushi chickens. The dermal shank pigmentation was not present in the one-day-old Gushi chickens. Additionally, the results of the association analysis and the expression analysis showed that GRAMD3 could be the most likely candidate gene for the Id locus. However, we did not detect a mutation, i.e. significantly associated with this trait within GRAMD3. Therefore, we concluded that the variations located in the flanking region of GRAMD3 led to the abnormal expression of GRAMD3, which requires further study.
机译：皮肤小腿色素沉着是鸡品种的定义特征之一，是由黑色素在小腿真皮中的异常沉积引起的。异常沉积是由性别相关的真皮黑色素（Id）抑制剂控制的。在这项研究中，我们旨在通过关联分析和差异表达分析来定位负责鸡真皮小腿色素沉着的基因。根据我们的结果，选择了位于Z染色体（chrZ）：71–73 Mb（galGal3）中的72个单核苷酸多态性（SNP），以进一步研究它们与96固始母鸡和96固始母鸡纯系中皮肤小腿色素沉着的关系。母鸡皮肤黄黄色。关联分析的结果表明，位于chrZ：72.58–72.99 Mb（galGal3）（chrZ：79.02–79.44 Mb（galGal4））中的SNP与真皮小腿色素沉着密切相关。根据我们以前的研究结果和当前的关联分析，得出锌指蛋白608（ZNF608），包含3的GRAM结构域（GRAMD3），醛脱氢酶7家族成员A1（ALDH7A1），fem-1同源物C（FEM1C）分别选择beta-1,4-半乳糖基转移酶1（B4GALT1）和versican（VCAN）基因进行差异表达分析。基因表达谱表明，在以真皮小腿色素沉着为特征的350日龄固始鸡中，小腿真皮组织中GRAMD3基因的表达显着高于（P = 0.010738 <0.05），而在一天长的固始鸡中则更高。在一日大的固始鸡中不存在真皮小腿色素沉着。另外，关联分析和表达分析的结果表明GRAMD3可能是Id基因座的最可能候选基因。然而，我们没有检测到突变，即与GRAMD3中的该性状显着相关。因此，我们得出的结论是，位于GRAMD3侧翼区域的变异导致GRAMD3的异常表达，需要进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号