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首页> 外文期刊>Journal of genetics >Mapping of Id locus for dermal shank melanin in a Chinese indigenous chicken breed
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Mapping of Id locus for dermal shank melanin in a Chinese indigenous chicken breed

机译：在中国土着鸡品种中的皮肤柄黑素蛋白的ID基因座的映射

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摘要

The dermal shank pigmentation, one of the defining traits of chicken breeds, is caused by an abnormal deposition of melanin in the dermis of the shank. The abnormal deposition is controlled by the sex-linked inhibitor of dermal melanin (Id). In this study, we aim to locate the gene responsible for the dermal shank pigmentation in chickens by an association analysis and a differential expression analysis. Based on our results, 72 single-nucleotide polymorphisms (SNPs) located in Z chromosome (chrZ): 71-73 Mb (galGal3) were selected to further explore their relationships with the dermal shank pigmentation in pure lines of 96 Gushi hens and 96 Gushi hens with a yellow shank skin colour. The results of the association analysis showed that the SNPs located in chrZ: 72.58-72.99 Mb (galGal3) (chrZ: 79.02-79.44 Mb (galGal4)) are significantly associated with the dermal shank pigmentation. Based on the results of our previous studies and the present association analysis, the zinc-finger protein 608 (ZNF608), GRAM domain containing 3 (GRAMD3), aldehyde dehydrogenase 7 family member A1 (ALDH7A1), fem-1 homologue C (FEM1C), beta-1,4-galactosyltransferase 1 (B4GALT1) and versican (VCAN) genes were selected for the differential expression analysis. The gene expression profiles showed that the expression of GRAMD3 gene in the dermis tissues of the shank was significantly () higher in 350-day-old Gushi chickens characterized by the dermal shank pigmentation than in one-day-old Gushi chickens. The dermal shank pigmentation was not present in the one-day-old Gushi chickens. Additionally, the results of the association analysis and the expression analysis showed that GRAMD3 could be the most likely candidate gene for the Id locus. However, we did not detect a mutation, i.e. significantly associated with this trait within GRAMD3. Therefore, we concluded that the variations located in the flanking region of GRAMD3 led to the abnormal expression of GRAMD3, which requires further study.
机译：皮肤柄色素沉着是鸡品种的定义性状之一，是由黑色素在柄的真皮中的异常沉积引起的。异常沉积由皮肤混合物（ID）的性关系抑制剂控制。在这项研究中，我们的目标是通过关联分析和差异表达分析定位负责鸡中皮肤柄色素沉着的基因。基于我们的结果，选择位于Z染色体（CHRZ）的72种单核苷酸多态性（SNP）：71-73MB（Galgal3），进一步探讨其与96甘氏母鸡96岁纯线的皮肤柄色素沉着的关系母鸡带着黄色柄肤色。关联分析的结果表明，位于CHRZ：72.58-72.99 MB（Galgal3）中的SNP（Chrz：79.02-79.44 MB（Galgal4））与真皮柄色素沉着显着相关。基于我们之前的研究结果和本关联分析，锌 - 手指蛋白608（ZNF608），含有3（克仑3）的克域，醛脱氢酶7家族成员A1（ALDH7A1），FEM-1同源物C（FEM1C）选择β-1,4-半乳糖基转移酶1（B4GALT1）和Versican（VCAN）基因用于差异表达分析。基因表达谱表明，在350天古老的甘露鸡中的350天老甘蓝鸡中的皮肤组织中克拉姆3基因的表达显着（）高于皮肤柄色素沉着的血小杂鸡。皮肤柄色素沉着不存在于一天古老的甘水鸡中。另外，关联分析的结果和表达分析表明，Gramd3可能是ID基因座的最可能候选基因。然而，我们没有检测到突变，即在GRAMD3中与这种特征显着相关。因此，我们得出结论，位于克拉姆3的侧翼区域中的变化导致克拉姆3的异常表达，这需要进一步研究。

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