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首页> 外文期刊>Journal of genetics >Characterization and expression of Xiphophorus maculatus microsatellite Msb069 full sequence in subgenus Poecilia
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Characterization and expression of Xiphophorus maculatus microsatellite Msb069 full sequence in subgenus Poecilia

机译：中华绒螯蟹亚种Xiphophorus maculatus微卫星Msb069全序列的表征和表达

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Msb069 primer pairs encompassed region is believed to be associated with a quantitative trait loci (QTL) of dorsal fin length in subgenus Poecilia. However, detailed investigation on Msb069 which originated from Xiphophorus on subgenus Poecilia remains unexplored. In this study, full sequence of Msb069 was characterized by sequencing bioinformatics analysis and gene expression. The sequence analysis of Msb069 primer pairs encompassed region on three species of Poecilia revealed higher number of microsatellite tandem repeats in Poecilia latipinna (ATG16) compared to P. sphenops (ATG13a??14). There is no notable pattern of ATGtandem repeats discovered in the hybrids. The full sequence of Msb069 is 734 bp in length and showed a 233 bp conserved region between Xiphophorus and Poecilia. BLAST search performed on this sequence revealed no significant similarities. Nonquantitative RT-PCR exhibited the presence of Msb069 transcripts in three different tissues in subgenus Poecilia. Meanwhile, quantitative RTPCR expression on two different tissues showed relatively higher expression of Msb069 transcript in P. latipinna dorsal fin tissues inboth male and female fishes, suggesting a repressive function of this transcript with respect to dorsal fin length. However the exact gene expression event of Msb069 is still unknown and requires further investigation.
机译：Msb069引物对涵盖的区域被认为与Poecilia亚属的背鳍长度的数量性状基因座（QTL）相关。但是，尚没有对起源于Poecilia亚属的Xiphophorus的Msb069进行详细研究。在这项研究中，通过测序生物信息学分析和基因表达来表征Msb069的全序列。 Msb069引物对的序列分析涵盖了三种Poecilia物种的区域，显示与P. sphenops（ATG13a ?? 14）相比，Poecilia latipinna（ATG16）中微卫星串联重复序列的数量更高。在杂种中没有发现明显的ATG串联重复序列。 Msb069的完整序列长度为734 bp，并在Xiphophorus和Poecilia之间显示了233 bp的保守区域。在该序列上进行的BLAST搜索没有发现明显的相似性。非定量RT-PCR显示在Poecilia亚属的三个不同组织中存在Msb069转录本。同时，在两种不同组织上的定量RTPCR表达表明，在雄性和雌性鱼类的拉氏顶鳍背鳍组织中，Msb069转录物的表达相对较高，表明该转录物对背鳍长度具有抑制作用。但是，尚不清楚Msb069的确切基因表达事件，需要进一步研究。

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