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首页> 外文期刊>Journal of Experimental Neuroscience >Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach:
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Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach:

机译：使用蛋白质组学方法探索脊髓衰老相关蛋白：

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How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging–related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting. Among the screened proteins, we selected α-crystallin B-subunit (αB-crystallin) and peripherin for further investigation because these proteins were previously reported to be related to central nervous system pathologies. Immunohistochemistry and Western blotting revealed significant upregulation of αB-crystallin and peripherin expression in aged rat spinal cord. Further exploration is needed to elucidate the precise mechanism and potential role of these upregulated proteins in spinal cord aging processes.
机译：衰老如何在分子水平上影响脊髓尚不清楚。这项研究的目的是探讨与脊髓衰老相关的蛋白质，这些蛋白质可能与脊髓衰老相关的病理机制有关。从动物中解剖出2岁和8周龄的雌性Sprague-Dawley大鼠的脊髓。对蛋白质样品进行2-维聚丙烯酰胺凝胶电泳，然后进行质谱分析。通过免疫组织化学和蛋白质印迹进一步研究筛选的蛋白质。在筛选的蛋白质中，我们选择了α-晶状蛋白B亚基（αB-晶状蛋白）和外围蛋白进行进一步研究，因为先前已报道这些蛋白与中枢神经系统病理相关。免疫组织化学和Western印迹显示老年大鼠脊髓中αB-晶状体蛋白和外周蛋白表达显着上调。需要进一步探索以阐明这些上调蛋白在脊髓衰老过程中的确切机制和潜在作用。

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