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首页> 外文期刊>Journal of Ginseng Research >Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells
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Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells

机译：人参皂苷Rb1对他克莫司诱导的肾小管LLC-PK1细胞凋亡的保护作用

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Background The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells. Methods LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay. Results Reduction in cell viability by 60μM FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 (10μM and 50μM). Conclusion The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.
机译：背景本研究的目的是评估从人参中分离出的六种人参皂苷（Rb1，Rb2，Rc，Rd，Rg1和Rg3）对他克莫司（FK506）诱导的肾小管LLC-PK1凋亡的潜在保护作用。细胞。方法用FK506和人参皂苷处理LLC-PK1细胞，测定细胞活力。通过蛋白质印迹分析评估促分裂原活化蛋白激酶，caspase-3和肾损伤分子1（KIM-1）的蛋白表达。使用基于图像的细胞计数测定法测量凋亡细胞的数目。结果与人参皂苷Rg1和Rb1共同处理可明显改善60μMFK506的细胞活力。在用FK506处理的LLC-PK1细胞中，p38，细胞外信号调节激酶和KIM-1的磷酸化以及caspase-3的裂解显着增加，而与人参皂甙Rb1共同处理后，p38的磷酸化显着降低。人参皂苷Rb1（10μM和50μM）共同处理后，凋亡细胞的数量减少了6.0％。结论人参皂苷Rb1对FK506诱导的细胞凋亡具有抗凋亡作用，其作用是通过抑制丝裂原活化的蛋白激酶和激活caspase来实现的。

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