• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Digest Journal of Nanomaterials and Biostructures >FUSIONOF RAW 264.7 MACROPHAGE CELLSONMICRO SCALE FINE PILLAR PATTERN S
【24h】

FUSIONOF RAW 264.7 MACROPHAGE CELLSONMICRO SCALE FINE PILLAR PATTERN S

机译：RAW 264.7巨噬细胞微尺度精细柱状图样的融合

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Macrophage dependent immune response to the implant surface sometimes results infailure of implant. M acrophage behaviors are sensitive to the topological factors involvingmicroano patterns on the implant s urface . However , further investigations aboutmacrophage behaviors toward the fine microano patterns on the implant surface arerequired. In the present study, we investigated the effect s of fine micro scale pillar pattern sfound on the surface of implants on macrophage fusion. Micro pillar s with dia meters of 2μm, 1 μm, and 500 nm and a height of 500 nm were fabricated by nanoimprinting on acyclo olefin polymer (COP) film. RAW 264.7 macrophages attachment assay show s that 1μm and 500 nm pillar s improved cell attachment than the planar surface. Macrophagefusion assay shows that the number of multi nucleated cell s on 1 μm and 500 nm pillar swas approximately 1. 4 to 1.5 times higher than that on the planar surface . However, 2μm pillar was ineffective in both the assays Interestingly, the expression of F actin andvinculin in mononuclear and multinucleated cells on the pillar s appeared ring shaped alongthe circumference of pillars , indicating frustrated phagocytosis . We suggest that finemicro patterning of the substrate could be one of th e factor s that control cell attachmentand fusion of RAW 264.7 cells.
机译：巨噬细胞对植入物表面的免疫反应有时会导致植入物失败。巨噬细胞的行为对涉及植入物表面微观/纳米图案的拓扑因素敏感。然而，需要进一步研究巨噬细胞对植入物表面上微细/纳米图案的行为。在本研究中，我们调查了在植入物表面上发现的微小的微型柱状图案对巨噬细胞融合的影响。通过在非环烯烃聚合物（COP）膜上进行纳米压印，制造了直径分别为2μm，1μm和500nm，高度为500nm的微柱。 RAW 264.7巨噬细胞附着试验显示1μm和500 nm柱比平表面改善了细胞附着。巨噬细胞融合试验表明，1μm和500 nm柱上的多核细胞数比平面数高1. 4至1.5倍。然而，2μm的柱子在两种试验中均无效。有趣的是，F肌动蛋白和Vinculin在柱子的单核和多核细胞中的表达沿柱子的圆周呈环形，表明吞噬作用受阻。我们认为底物的精细图案化可能是控制细胞附着和RAW 264.7细胞融合的因素之一。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号