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首页> 外文期刊>Journal of Human Reproductive Sciences >Blastocyst cryopreservation using solid surface vitrification: A preliminary study
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Blastocyst cryopreservation using solid surface vitrification: A preliminary study

机译：固态表面玻璃化冷冻囊胚的初步研究

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OBJECTIVE:The objective was to evaluate the effectiveness of a blastocyst cryopreservation program using solid surface vitrification.SETTING:This study took place in a university teaching hospital.STUDY DESIGN:Retrospective observational study.MATERIALS AND METHODS:Women undergoing frozen embryo transfer cycles over a 4-year period between 2006 and 2010 were studied. The cryopreservation policy followed was a vitrification protocol performed at the blastocyst stage, using a solid surface (nonimmersion) method. The post-thaw survival rate, implantation rate, clinical pregnancy rate, live birth rate, and neonatal outcome were recorded.RESULTS:Eighty-one women underwent 86 frozen embryo transfer cycles. Of the 240 blastocysts warmed, 204 survived giving a cryosurvival rate of 85% (204/240). The clinical pregnancy, implantation, miscarriage, ongoing pregnancy, and live birth rates per transfer were 47%, 29%, 12%, 16%, and 23% respectively. Of the 20 live births, there were 16 singletons and 4 twins. Eleven boys and 13 girls were delivered with no major or minor abnormality detected.CONCLUSION(S):The blastocyst vitrification protocol using the solid surface method is effective with results comparable to fresh blastocyst transfers. While retaining the rapid cooling effect, the nonimmersion technique eliminates the risk of contamination and disease transmission. Larger studies with long-term follow-up data would further confirm the efficacy and safety of this method of vitrification.
机译：目的：目的是评估使用固态表面玻璃化进行的囊胚冷冻保存程序的有效性。背景：这项研究是在一家大学教学医院进行的。研究设计：回顾性观察性研究。材料与方法：妇女在超过一个月的时间里经历了冷冻胚胎移植周期研究了2006年至2010年的4年时间。遵循的冷冻保存策略是在囊胚阶段使用固体表面（非浸没）方法执行玻璃化方案。记录解冻后的存活率，着床率，临床妊娠率，活产率和新生儿结局。结果：81例妇女经历了86次冷冻胚胎移植周期。在加热的240个胚泡中，有204个存活，冷冻存活率为85％（204/240）。每次转移的临床妊娠率，植入率，流产率，持续妊娠率和活产率分别为47％，29％，12％，16％和23％。在20个活产婴儿中，有16个单胎和4个双胞胎。分娩的男孩和13名女孩没有重大或次要异常。结论：使用固体表面方法进行囊胚玻璃化的方法是有效的，其结果可与新鲜囊胚转移相媲美。在保持快速冷却效果的同时，非浸入式技术消除了污染和疾病传播的风险。具有长期随访数据的较大研究将进一步证实这种玻璃化方法的有效性和安全性。

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