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首页> 外文期刊>Journal of International Medical Research >Human Papillomavirus Type 16 Exists in Bacteria Isolated from Cervical Cancer Biopsies
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Human Papillomavirus Type 16 Exists in Bacteria Isolated from Cervical Cancer Biopsies

机译：从宫颈癌活检组织分离的细菌中存在16型人乳头瘤病毒。

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This study investigated the association between infectious microbes and persistent infection with human papillomavirus type 16 (HPV-16) in cervical cancer. Bacterial strains (identified as Enterococcus, Staphylococcus, Bacillus and Corynebacterium, based on their partial 16S rDNA sequence) were HPV-16 positive from 12 out of 14 cervical cancer biopsies. Total DNA was isolated from the four bacterial strains, and HPV-16 genes and genome were detected using polymerase chain reaction (PCR) and Southern blotting. RNA transcripts for HPV-16 E6 and L1 genes were detected in total bacterial RNA samples using reverse transcription-PCR, and HPV-16 L1 protein expression was detected in bacterial cells by Western blotting and immunocolloidal gold electron microscopy. The presence of virus particles in bacterial cells was demonstrated by transmission electron microscopy. The results suggest that bacteria carrying HPV-16 could provide a potential explanation for how infectious microbes contribute to the progression from HPV-16 infection to cervical cancer.
机译：这项研究调查了宫颈癌中传染性微生物与16型人乳头瘤病毒（HPV-16）持续感染之间的关系。细菌菌株（根据它们的部分16S rDNA序列鉴定为肠球菌，葡萄球菌，芽孢杆菌和棒状杆菌）在14例宫颈癌活检中有12例是HPV-16阳性。从四个细菌菌株中分离出总DNA，并使用聚合酶链反应（PCR）和Southern印迹检测HPV-16基因和基因组。使用逆转录PCR在总细菌RNA样品中检测到HPV-16 E6和L1基因的RNA转录本，并通过蛋白质印迹和免疫胶体金电子显微镜在细菌细胞中检测到HPV-16 L1蛋白的表达。通过透射电子显微镜证实细菌细胞中病毒颗粒的存在。结果表明，携带HPV-16的细菌可以为感染性微生物如何促进从HPV-16感染到宫颈癌的发展提供潜在的解释。

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