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首页> 外文期刊>Journal of Indian Society of Periodontology >Microbial contamination of contact lenses after scaling and root planing using ultrasonic scalers with and without protective eyewear: A clinical and microbiological study
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Microbial contamination of contact lenses after scaling and root planing using ultrasonic scalers with and without protective eyewear: A clinical and microbiological study

机译：使用和不使用防护眼镜的超声洁牙机刮牙和根部刨光后接触镜的微生物污染：一项临床和微生物学研究

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Background: Ultrasonic scaler is a preferential treatment modality among the clinicians. However, the aerosol/splatter generated is a concern for patients and practitioners. Therefore, the purpose of this study was to evaluate contamination of contact lenses of the dentist after scaling and root planing using ultrasonic scalers with and without protective eyewear. Materials and Methods: Thirty patients were randomly selected for scaling and root planing and divided into 2 groups of 15 each. Group A - dentist wearing contact lenses and protective eyewear. Group B - dentist wearing only contact lenses. After scaling and root planing using ultrasonic scalers, the lenses were subjected to culture and 16S rRNA (16S ribosomal RNA) gene sequencing. Results: In Group A – 15 out of thirty samples were contaminated, in Group B – all the thirty samples were contaminated. Most of the samples showed Gram-positive bacteria and 5 samples were contaminated with fungi. 16S rRNA gene sequencing of forty contaminated samples showed that 31 were contaminated with Streptococcus mutans and 9 with Staphylococcus aureus. Conclusion: Keeping in mind the limitation of the study for the absence of negative control, we would like to conclude that dental practitioners should better avoid contact lenses in a dental setup because of the risk of contamination of the contact lenses from the various dental procedures which can produce aerosol/splatter and if worn, it is recommended to wear protective eyewear.
机译：背景：超声洁牙机是临床医生中的一种优先治疗方式。然而，产生的气溶胶/飞溅物是患者和从业者的关注点。因此，本研究的目的是评估在使用和不使用防护眼镜的情况下，使用超声波洁牙机进行洁牙和根部平整后，牙医对隐形眼镜的污染程度。材料和方法：随机选择30例患者进行结垢和根部平整，分为2组，每组15个。 A组-牙医戴隐形眼镜和防护眼镜。 B组-牙医仅戴隐形眼镜。在使用超声洁牙机进行洁牙和根部平整之后，对镜片进行培养和16S rRNA（16S核糖体RNA）基因测序。结果：在A组中-三十个样本中有15个被污染，在B组中-三十个样本都被污染。大多数样品显示出革兰氏阳性菌，有5个样品被真菌污染。对40个受污染样品的16S rRNA基因测序表明，变形链球菌污染了31个，金黄色葡萄球菌污染了9个。结论：考虑到缺乏阴性对照的研究局限性，我们想得出的结论是，牙科医生应在牙科设备中更好地避免使用隐形眼镜，因为各种牙科程序会污染隐形眼镜，会产生气溶胶/飞溅物，如果戴了，建议戴防护眼镜。

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