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首页> 外文期刊>Journal of International Medical Research >Direct Detection of Bordetella pertussis and Bordetella parapertussis: Comparison of Polymerase Chain Reaction and Culture
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Direct Detection of Bordetella pertussis and Bordetella parapertussis: Comparison of Polymerase Chain Reaction and Culture

机译：百日咳博德特氏菌和副百日咳博德特氏菌的直接检测：聚合酶链反应和培养的比较

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摘要

We evaluated the diagnostic performance of a genomic DNA amplification method for Bordetella pertussis and Bordetella parapertussis compared with culture isolation. Aliquots from B. pertussis and B. parapertussis cultures were added to sterile physiological saline or sterile distilled water to give bacterial suspensions of 108 cells/ml and serial dilutions were prepared. Suspensions in physiological saline were cultured on charcoal agar medium; bacterial growth was observed up to dilutions of 10?7. Suspensions in distilled water were subjected to DNA extraction and nested polymerase chain reaction (PCR) was performed on the extracts; the PCR was positive up to dilutions of 10?8 for B. pertussis and 10?9 for B. parapertussis. Since the efficacy of culture isolation, regarded as the standard for the detection of B. pertussis and B. parapertussis, declines after the first stage of pertussis or with prior vaccination or antibiotic therapy, PCR, although not yet standardized, may provide an alternative diagnostic tool.
机译：我们比较了百日咳博德特氏菌和副百日咳博德特氏菌的基因组DNA扩增方法与培养物分离的诊断性能。将百日咳博德特氏菌和百日咳博德特氏菌培养物的等分试样添加到无菌生理盐水或无菌蒸馏水中，以得到108个细胞/ ml的细菌悬浮液，并制备系列稀释液。在木炭琼脂培养基上培养生理盐水中的悬浮液;观察到细菌生长直至稀释至10-7。对蒸馏水中的悬浮液进行DNA提取，并对提取物进行巢式聚合酶链反应（PCR）;在百日咳博德特氏菌和副百日咳博德特氏菌的稀释度分别为10-8和10-9的情况下，PCR呈阳性。由于作为百日咳博德特氏菌和副百日咳博德特氏菌的检测标准的分离培养物的功效在百日咳的第一阶段后或在预先接种疫苗或进行抗生素治疗后下降，因此，PCR（尽管尚未标准化）可提供另一种诊断方法工具。

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