• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of International Dental and Medical Research >The Effect of Mahkota Dewa (Phaleria Macrocarpa L.) Leaf Extract Encapsulated in Chitosan Nanoparticles on iNOS and COX-2 Expression in Dextran Sodium Sulphate-Induced Colitis Mice Model
【24h】

The Effect of Mahkota Dewa (Phaleria Macrocarpa L.) Leaf Extract Encapsulated in Chitosan Nanoparticles on iNOS and COX-2 Expression in Dextran Sodium Sulphate-Induced Colitis Mice Model

机译：壳聚糖纳米粒包裹的Mahkota Dewa（Phaleria Macrocarpa L.）叶提取物对葡聚糖硫酸钠诱导的结肠炎小鼠模型中iNOS和COX-2表达的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mahkota Dewa (Phaleria macrocarpa L) is indigenous shrub found in Indonesia commonly used in traditional medicine for its anti-inflammatory effects. This study was conducted to assay Mahkota Dewa leaf extract packaged in chitosan nanoparticles provides superior anti-inflammatory effects on ulcerative colitis induced by dextran sodium sulphate (DSS) compared to Mahkota Dewa leaf extract alone. This study used 36 mice divided into 6 groups: normal, negative control, leaf extract dose 12.5 mg, leaf extract dose 25 mg, leaf extract chitosan nanoparticle dose 6.25 mg, and leaf extract chitosan nanoparticle dose 12.5 mg. Colitis was induced by administering DSS (2 % w/v) through drinking water at 1, 3, and 5 weeks of treatment. At the end of the study, colon samples were taken from the mice for immunohistochemical analysis; the anti-inflammatory effects of each extract were evaluated by determining the expression of cyclooxygenase-2 (COX-2) and induducible mitric oxide synthase (iNOS) score 0 - 4 in the mouse colon using the image G profiler method. Mice administered Mahkota Dewa leaf extract at doses of 12.5 and 25 mg and Mahkota Dewa leaf extract in chitosan nanoparticles at doses of 6.25 and 12.5 mg all demonstrated significantly decreased expression of COX-2 and iNOS (p < 0.001). No significant differences between Mahkota Dewa leaf extract and Mahkota Dewa leaf extract in chitosan nanoparticles were observed. Mahkota Dewa leaf extract and Mahkota Dewa leaf extract in chitosan nanoparticles are equally effective at suppressing COX-2 and iNOS expression in mice with DSS-induced colitis.
机译：Mahkota Dewa（Phaleria macrocarpa L）是在印度尼西亚发现的土生灌木，由于其抗炎作用而常用于传统医学。进行这项研究是为了测定包装在壳聚糖纳米颗粒中的Mahkota Dewa叶提取物与单独使用Mahkota Dewa叶提取物相比，对葡聚糖硫酸钠（DSS）诱发的溃疡性结肠炎具有卓越的抗炎作用。这项研究使用了36只小鼠，分为6组：正常，阴性对照，叶提取物剂量12.5 mg，叶提取物剂量25 mg，叶提取物壳聚糖纳米颗粒剂量6.25 mg和叶提取物壳聚糖纳米颗粒剂量12.5 mg。在治疗的1、3和5周时通过饮水给予DSS（2％w / v）诱发结肠炎。在研究结束时，从小鼠中采集结肠样本进行免疫组织化学分析;通过使用图像G轮廓分析法确定小鼠结肠中环氧合酶2（COX-2）的表达和诱导型三氧化二合酶（iNOS）得分0-4来评估每种提取物的抗炎作用。小鼠分别以12.5和25 mg的剂量服用Mahkota Dewa叶提取物以及6.25和12.5 mg剂量的壳聚糖纳米颗粒中的Mahkota Dewa叶提取物均证明COX-2和iNOS的表达显着降低（p <0.001）。在壳聚糖纳米颗粒中未观察到Mahkota Dewa叶提取物和Mahkota Dewa叶提取物之间的显着差异。壳聚糖纳米颗粒中的Mahkota Dewa叶提取物和Mahkota Dewa叶提取物在抑制DSS诱发的结肠炎小鼠中的COX-2和iNOS表达方面同样有效。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号