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首页> 外文期刊>Journal of King Saud University >Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei
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Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei

机译：双链RNA对白斑综合症病毒（WSSV）非结构性（ orf89 ， wsv191 ）和结构性（南美白对虾中的italic> vp28 ， vp26 ）基因

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White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. RNA interference (RNAi) is a promising tool against viral infections. Previous works with RNAi showed different antiviral efficacies depending on the silenced gene. This work evaluated the antiviral efficacy of double-stranded (ds) RNA against two non-structural ( orf89 , wsv191 ) WSSV genes compared to structural ( vp26 , vp28 ) genes to inhibit an experimental WSSV infection. Gene orf89 encodes a putative regulatory protein and gene white spot virus ( wsv ) 191 encodes a nonspecific nuclease; whereas genes vp26 and vp28 encode envelope proteins, respectively. Molecules of dsRNA against each of the WSSV genes were intramuscularly injected (4 μg per shrimp) into a group of shrimp 48 h before a WSSV challenge. The highest antiviral activity occurred with dsRNA against orf89 , vp28 and vp26 (cumulative mortalities 10%, 10% and 21%, respectively). In contrast, the least effective treatment was wsv191 dsRNA (cumulative mortality 83%). All dead animals were WSSV-positive by one-step PCR, whereas reverse-transcription PCR of all surviving shrimp confirmed inhibition of virus replication. This study showed that dsRNA against WSSV genes orf89 , vp28 and vp26 were highly effective to inhibit virus replication and suggest an essential role in WSSV infection. Non-structural WSSV genes such as orf89 can be used as novel targets to design therapeutic RNAi molecules against WSSV infection.
机译：白斑综合症病毒（WSSV）是虾养殖中的主要病原体。 RNA干扰（RNAi）是对抗病毒感染的有前途的工具。 RNAi的先前工作显示了不同的抗病毒功效，具体取决于沉默的基因。这项工作评估了双链（ds）RNA对两个非结构（orf89，wsv191）WSSV基因与结构（vp26，vp28）基因的抗病毒功效，以抑制实验性WSSV感染。基因orf89编码一个假定的调节蛋白，基因白斑病毒（wsv）191编码一个非特异性核酸酶。而基因vp26和vp28分别编码包膜蛋白。在WSSV攻击前48小时，将针对每个WSSV基因的dsRNA分子肌肉注射（每只虾4μg）到一组虾中。 dsRNA对orf89，vp28和vp26的抗病毒活性最高（累计死亡率分别为10％，10％和21％）。相反，最无效的治疗方法是wsv191 dsRNA（累积死亡率83％）。通过一步PCR，所有死亡的动物均为WSSV阳性，而所有存活虾的逆转录PCR证实了病毒复制的抑制。这项研究表明，针对WSSV基因orf89，vp28和vp26的dsRNA在抑制病毒复制方面非常有效，并暗示了在WSSV感染中的重要作用。非结构化WSSV基因（例如orf89）可用作设计针对WSSV感染的治疗性RNAi分子的新型靶标。

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