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首页> 外文期刊>Journal of King Saud University >Simultaneous HPLC quantitative analysis of mangostin derivatives in Tetragonula pagdeni propolis extracts
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Simultaneous HPLC quantitative analysis of mangostin derivatives in Tetragonula pagdeni propolis extracts

机译：高效液相色谱法同时定量 Tetragonula pagdeni 蜂胶提取物中的芒果素衍生物

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摘要

Propolis has been used as indigenous medicine for curing numerous maladies. The one that is of ethnopharmacological use is stingless bee propolis from Tetragonula pagdeni . A simultaneous high-performance liquid chromatography (HPLC) investigation was developed and validated to determine the contents of bioactive compounds: 3-isomangostin, gamma-mangostin, beta-mangostin, and alpha-mangostin. HPLC analysis was effectively performed using a Hypersil BDS C 18 column, with the gradient elution of methanol–0.2% formic acid and a flow rate of 1 ml/min, at 25 °C and detected at 245 nm. Parameters for the validation included accuracy, precision, linearity, and limits of quantitation and detection. The developed HPLC technique was precise, with lower than 2% relative standard deviation. The recovery values of 3-isomangostin, gamma-mangostin, beta-mangostin, and alpha-mangostin in the extracts were 99.98%, 99.97%, 98.98% and 99.19%, respectively. The average contents of these mixtures in the propolis extracts collected from different seasons were 0.127%, 1.008%, 0.323% and 2.703% (w/w), respectively. The developed HPLC technique was suitable and practical for the simultaneous analysis of these mangostin derivatives in T. pagdeni propolis and would be a valuable guidance for the standardization of its pharmaceutical products.
机译：蜂胶已被用作治疗多种疾病的本土药物。种族药理学用途的一种是来自Tetragonula pagdeni的无刺蜂胶。同时进行了高效液相色谱（HPLC）研究并进行了验证，以确定生物活性化合物的含量：3-异芒果素，γ-芒果素，β-芒果素和α-芒果素。使用Hypersil BDS C 18色谱柱可有效地进行HPLC分析，在25°C且于245 nm处检测到甲醇–0.2％甲酸的梯度洗脱和1 ml / min的流速。验证的参数包括准确性，准确性，线性度以及定量和检测极限。所开发的HPLC技术非常精确，相对标准偏差低于2％。提取物中3-异mangostin，γ-mangostin，β-mangostin和α-mangostin的回收率分别为99.98％，99.97％，98.98％和99.19％。在不同季节收集的蜂胶提取物中，这些混合物的平均含量分别为0.127％，1.008％，0.323％和2.703％（w / w）。发达的HPLC技术适用于同时分析帕格登尼蜂胶中的这些芒果苷衍生物，并且对于对其药物产品的标准化提供有价值的指导。

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