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首页> 外文期刊>Journal of King Saud University >Isolation and identification of chromium reducing bacteria from tannery effluent
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Isolation and identification of chromium reducing bacteria from tannery effluent

机译:制革废水中铬还原菌的分离与鉴定

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Industrial wastes are capable of polluting the soil and groundwater. The tanning industry is considered as a potential environmental polluting agent through out the world. Chromium (Cr), a toxic heavy metal, is a major constituent of tannery waste, and its accumulation in soil and water causes serious environmental issues of increasing public concern in India particularly in Tamil Nadu. The present study deals with isolation and identification of chromium reducing bacteria isolated from tannery effluent collected from Crompet, Chennai, Tamil Nadu (latitude 12°57′51.8″N and longitude 80°07′58.1″E). Two chromium reducing bacteria were isolated; and the identification of the isolates were done by 16 s rRNA sequencing followed by BLAST results asKlebsiella pneumoniaeandMangrovibacter yixingensis.Furthermore the presence of chromium reductase gene in the isolated bacteria was confirmed by PCR techniques. Bioremediation using bacteria may provide an alternative or aid conventional methods of metal removal/metal recovery. The newly identified chromium reducing bacteria would be useful for the detoxification of heavy metal contaminated tannery effluent and metal recovery in mining process.
机译:工业废料能够污染土壤和地下水。制革业被认为是全世界潜在的环境污染介质。铬(Cr)是有毒的重金属,是制革厂废物的主要成分,其在土壤和水中的积累引起严重的环境问题,在印度,尤其是在泰米尔纳德邦,引起了越来越多的公众关注。本研究涉及分离和鉴定从克伦佩,金奈,泰米尔纳德邦(北纬12°57′51.8” N和东经80°07′58.1” E)制革厂废水中分离的还原铬细菌。分离出两种还原铬的细菌;通过16 s rRNA测序和BLAST结果鉴定出分离株,分别为肺炎克雷伯菌和宜兴曼氏杆菌。此外,通过PCR技术确认了分离的细菌中铬还原酶基因的存在。使用细菌的生物修复可以提供替代方法或辅助常规的金属去除/金属回收方法。新近发现的还原铬细菌可用于对重金属污染的制革厂废水进行排毒,并在采矿过程中回收金属。

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