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首页> 外文期刊>Journal of King Saud University >C677T genotypes in methyltetrahydrofolate reductase gene in student obesity
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C677T genotypes in methyltetrahydrofolate reductase gene in student obesity

机译：学生肥胖甲基四氢叶酸还原酶基因的C677T基因型

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Obesity is known as weight gaining programme which leads to increase in body mass index (BMI), is known for significant risk factors for chronic diseases. The prevalence of obesity has been rising in South Asian countries, specifically in Pakistan. Numerous studies have shown the association with metabolic diseases (ex; obesity) with single nucleotide polymorphisms. The present study has been designed to investigate the association between C677T polymorphism in methylenetetrahydrofolate reductase (MTHFR) gene with obesity in Pakistan population. In this case-control study, we have enrolled 200 obese students and 200 non-obese or control students based on the BMI. Genomic DNA was isolated with enrolled 400 blood samples and PCR was performed with C677T polymorphism in theMTHFRgene to analyse the frequency. Later on restriction enzyme length polymorphism analysis was carried out for the undigested PCR products. Genotyping frequencies for C677T polymorphism were compared between obesity and non-obesity students. Genotype, allele and various genetic model of inheritances were calculated and documented the significant association (p??0.05). The present study concludes C677T polymorphism inMTHFRgene was genetically associated between the obesity risks in Pakistan population. However, relation with Anova analysis showed the negative association with C677T polymorphism.
机译：肥胖被认为是导致体重指数（BMI）增加的体重增加计划，众所周知，肥胖是慢性疾病的重要危险因素。在南亚国家，尤其是在巴基斯坦，肥胖症的患病率一直在上升。大量研究表明，单核苷酸多态性与代谢性疾病（例如肥胖）有关。本研究旨在调查巴基斯坦人群中亚甲基四氢叶酸还原酶（MTHFR）基因的C677T多态性与肥胖之间的关系。在这个案例对照研究中，我们根据BMI招收了200名肥胖学生和200名非肥胖或对照学生。用400份血液样本分离基因组DNA，并在MTHFR基因中利用C677T多态性进行PCR以分析频率。随后，对未消化的PCR产物进行限制性酶长度多态性分析。比较了肥胖和非肥胖学生的C677T基因多态性的基因分型频率。计算基因型，等位基因和遗传的各种遗传模型，并记录其显着相关性（p <0.05）。但是，与肥胖症患者的年龄，体重和BMI等人体测量结果相比，我们用C677T多态性进行测量时，Anova分析无法证明其呈正相关（p≥0.05）。本研究得出结论，MTHFR基因中的C677T多态性与巴基斯坦人群的肥胖风险之间存在遗传相关性。然而，与Anova分析的关系显示出与C677T多态性的负相关。

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