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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >The role of G-CSF in mature neutrophil function is not related to GM-CSF-type cell priming.
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The role of G-CSF in mature neutrophil function is not related to GM-CSF-type cell priming.

机译：G-CSF在成熟的中性粒细胞功能中的作用与GM-CSF型细胞启动无关。

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Because of uncertainties regarding the comparability of granulocyte-macrophage and granulocyte colony-stimulating factors with regard to their effects on mature neutrophils (PMNs), we compared the actions of the two cytokines on reactive oxidant production and granular secretion by these cells. We found that chemiluminescence (CL) stimulated by formylmethionyl-leucyl-phenylalanine (fMLP) was not influenced by G-CSF (0.1-100 ng/ml), whereas GM-CSF priming (10 ng/ml) caused a nearly twofold increase in this PMN response. Moreover, the reactivity of PMNs treated with GM-CSF and G-CSF in combination was not different from that of PMNs treated with GM-CSF alone. GM-CSF (10 ng/ml) increased the rate of O2- production by 79%, caused a fivefold increase in fMLP-induced myeloperoxidase (MPO) secretion, and strongly enhanced CD11b expression. In contrast, G-CSF (50 ng/ml) only slightly increased O2- production (by 15%), and MPO secretion and CD11b expression remained unchanged. Both cytokines together gave results similar to those obtained with GM-CSF alone. In the presence of platelets (which by themselves enhanced PMN reactivity), the differences in the effects of the two cytokines persisted. We conclude that the priming effect of G-CSF on mature PMNs is negligible compared with that of GM-CSF. Our results are in conflict with previous reports of much more pronounced G-CSF effects but in accord with recent work showing the failure of this cytokine to induce a range of effects produced by GM-CSF. We therefore suggest that the primary role of G-CSF in mature PMN function is still unclear but may be related to the control of PMN distribution in view of the mobilizing and marginating effects of the cytokine in vivo.
机译：由于粒细胞巨噬细胞和粒细胞集落刺激因子对成熟嗜中性粒细胞（PMNs）的可比性尚不确定，因此我们比较了这两种细胞因子对这些细胞的反应性氧化剂产生和颗粒分泌的作用。我们发现甲酰甲硫酰基-亮氨酰-苯丙氨酸（fMLP）刺激的化学发光（CL）不受G-CSF（0.1-100 ng / ml）的影响，而GM-CSF引发（10 ng / ml）引起的化学发光增加了近两倍。这个PMN回应。而且，GM-CSF和G-CSF联合处理的PMN的反应性与单独GM-CSF处理的PMN的反应性没有区别。 GM-CSF（10 ng / ml）将O2产生的速率提高了79％，导致fMLP诱导的髓过氧化物酶（MPO）分泌增加了五倍，并大大增强了CD11b的表达。相反，G-CSF（50 ng / ml）仅略微增加了O2的产生（增加了15％），并且MPO分泌和CD11b表达保持不变。两种细胞因子共同产生的结果类似于单独使用GM-CSF获得的结果。在存在血小板的情况下（其自身增强了PMN反应性），两种细胞因子的作用差异仍然存在。我们得出的结论是，与GM-CSF相比，G-CSF对成熟PMN的启动作用微不足道。我们的结果与先前报道的更明显的G-CSF效应相矛盾，但与最近的研究表明该细胞因子不能诱导GM-CSF产生的一系列效应相矛盾。因此，我们建议，尚不清楚G-CSF在成熟PMN功能中的主要作用，但鉴于细胞因子在体内的动员和边缘化作用，可能与PMN分布的控制有关。

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