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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Gestation-related expression of the interferon-gamma receptor gene in mouse uterine and embryonic hematopoietic cells.
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Gestation-related expression of the interferon-gamma receptor gene in mouse uterine and embryonic hematopoietic cells.

机译：妊娠相关的干扰素-γ受体基因在小鼠子宫和胚胎造血细胞中的表达。

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Macrophages and natural killer (NK)-like cells are the major hematopoietic cell populations in the cycling and pregnant mouse uterus and are also found in the embryo. In order to evaluate potential receptivity of these cells to interferon-gamma (IFN-gamma), tissues taken from cycling and pregnant mice were tested for IFN-gamma receptor (IFN-gamma R) mRNA and protein. Macrophages were identified immunohistochemically by using the specific monoclonal antibody F4/80. NK cells were identified by their large size, distinctive intracellular granules, and binding of a monoclonal antibody to the common leukocyte antigen. In cycling uteri, the abundance of IFN-gamma R mRNA relative to an invariant message (glyceraldehyde-3-phosphate dehydrogenase) increased during progression of the hormonally regulated estrous cycle. IFN-gamma R mRNA in situ hybridization signals were slightly higher in macrophage-like than in other types of endometrial stromal cells. In pregnant uteri, the highest proportions of IFN-gamma R mRNA were observed at gestation day (g.d.) 16. Specific message and protein were present in uterine macrophages by g.d. 7 and in NK cells by g.d. 9. IFN-gamma R expression in both lineages remained stable through the balance of pregnancy. In embryos, IFN-gamma R mRNA increased between g.d. 14 and 16. Specific transcripts were present in many cells at g.d. 14, but none were detected in embryonic liver macrophages until g.d. 16. The results of this study suggest relationships between IFN-gamma R expression and ovarian hormones as well as cell maturation and support the postulate that IFN-gamma receptor-ligand interactions may improve the ability of uterine and embryonic hematopoietic cells to perform specific tasks during gestation.
机译：巨噬细胞和类自然杀伤（NK）细胞是循环和怀孕小鼠子宫中的主要造血细胞群体，并且也在胚胎中发现。为了评估这些细胞对干扰素-γ（IFN-γ）的潜在接受性，测试了从骑自行车和怀孕的小鼠身上获取的组织的IFN-γ受体（IFN-γR）mRNA和蛋白。通过使用特异性单克隆抗体F4 / 80免疫组化鉴定巨噬细胞。 NK细胞的大小，独特的细胞内颗粒以及单克隆抗体与常见白细胞抗原的结合得以鉴定。在子宫周期中，在激素调节的动情周期过程中，相对于不变的信息（甘油醛-3-磷酸脱氢酶），IFN-γR mRNA的含量增加。巨噬细胞样的IFN-γR mRNA原位杂交信号比其他类型的子宫内膜基质细胞高。在妊娠子宫中，在妊娠日观察到最高比例的IFN-γR mRNA。（g.d。）16。g.d在子宫巨噬细胞中存在特定的信息和蛋白质。 7和在NK细胞中按g.d. 9.通过妊娠平衡，两个谱系中的IFN-γR表达均保持稳定。在胚胎中，IFN-γRmRNA在g.d之间增加。图14和16显示了特定的转录本。 14，但直到胚胎肝巨噬细胞中未检测到。 16.这项研究的结果表明，IFN-γR表达与卵巢激素之间的关系以及细胞成熟，并支持IFN-γ受体-配体相互作用可能会改善子宫和胚胎造血细胞在此过程中执行特定任务的能力妊娠。

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