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首页> 外文期刊>Journal of Agricultural Science >In vitro Inhibition of Soilborn Phytopathogens Treated With Swine Wastewater
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In vitro Inhibition of Soilborn Phytopathogens Treated With Swine Wastewater

机译：猪废水处理对土壤源性植物病原菌的体外抑制作用

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Swine wastewater (SWW) is a residue from pig farming that presents a high load of nutrients and organic matter. The appliance of organic matter in soil alters the microbial dynamic and may suppress soilborn phytopathogens. This study aimed at evaluating the inhibition on mycelial growth of Sclerotinia sclerotiorum and Sclerotium rolfsii in vitro under SWW doses. Hereupon, three kilograms of a soil classified as red dystroferric latosol was collected and sieved. Half of it was autoclaved. SWW was incorporated at doses of 0 mL, 2.5 mL, 5 mL, 10 mL and 20 mL in both soil conditions, autoclaved and not autoclaved. Afterwards, 130 grams of each soil was separately put into Petri plates above what a thin layer (? 5 mL) of Water-Agar (2%) medium was carefully spread over. Above this agar layer, one disk (6 mm diameter) of pure mycelium from each fungal grown in Potato Dextrose Agar medium was individually placed on the center of each plate. Daily evaluations on mycelial growth measuring were taken and ended when mycelium in control plates (without SWW addition) reached plate borders. Results indicated that in autoclaved soil condition, the inhibition was proportional to the dose, what is to say that the higher the dose the less the mycelial growth. In not autoclaved soil there was no significant difference among treatments, suggesting stimuli on suppression effect for both pathogens caused by SWW. In addition, the confirmed potential of SWW as a suppressor of S. sclerotiorum and S. rolfsii leads to promising investigations on other phytopathogens hard to control.
机译：猪废水（SWW）是养猪场的残留物，具有很高的养分和有机质含量。土壤中有机物的迁移改变了微生物的动力学，并可能抑制土壤中的植物病原体。这项研究旨在评估在SWW剂量下体外对菌核菌和菌核菌的菌丝生长的抑制作用。因此，收集并筛分了三公斤被归类为红色的四铁酸黄腐溶胶的土壤。其中一半被高压灭菌。在两种土壤条件下，将SWW分别以0 mL，2.5 mL，5 mL，10 mL和20 mL的剂量进行高压灭菌和不高压灭菌。然后，将每种土壤130克分别放入培养皿中，在上面小心地铺一层薄薄的水-琼脂（2％）培养基（？5 mL）。在此琼脂层上方，将来自马铃薯右旋糖琼脂培养基中生长的每种真菌的纯菌丝的一个圆盘（直径为6 mm）分别放置在每个平板的中央。当对照板中的菌丝体（不添加SWW）到达板边界时，进行每日的菌丝生长测量评估并结束。结果表明，在高压灭菌的土壤条件下，抑制作用与剂量成正比，也就是说剂量越高，菌丝体生长越少。在未高压灭菌的土壤中，不同处理之间无显着差异，这表明刺激对SWW引起的两种病原体的抑制作用。此外，已证实的SWW可以作为S. sclerotiorum和S. rolfsii的抑制剂具有潜在的潜力，从而导致了对其他难以控制的植物病原体的有前途的研究。

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