• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of medical bacteriology. >Determination of Extended-Spectrum Beta-Lactamases Genes and Antibiotic Resistance Patterns in Escherichia coli Isolates from Healthy Cats
【24h】

Determination of Extended-Spectrum Beta-Lactamases Genes and Antibiotic Resistance Patterns in Escherichia coli Isolates from Healthy Cats

机译：健康猫大肠杆菌分离物中的广谱β-内酰胺酶基因和抗生素耐药性模式的确定

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: This study was set to detect extended-spectrum beta-lactamases (ESBLs) producing E. coli isolates and the genes underlying their resistance in relation to phylogenetic background from fecal samples of healthy owned cats.?Methods: A total of 50 E. coli isolates were confirmed by standard bacteriological tests. The phylogenetic analyses of the isolates were carried out by combinations of three genetic markers chuA, yjaA and DNA fragment TspE4.C2 by a triplex PCR method. The ESBL (blaCTXM, blaTEM, blaSHV, blaOXA) encoding genes were detected. To identify ESBL producing phenotypes, all selected isolates were screened with a double disk synergy test including cefotaxime, cefotaxime with clavulanic acid, ceftazidime and ceftazidime with clavulanic acid.?Results: Results showed that E. coli isolates fell into four phylogenetic groups (A, D, B1 and B2) with prevalence of 78%, 4%, 8%, 10% and five phylogenetic subgroups including A0 (74 %), A1 (4 %), B1 (8 %), B2–2 (6 %), B2–3 (4 %) and D1 (4 %), respectively. Among all E. coli isolates, 4% were positive for blaSHV, blaCTX-M-15 and blaOXA-1 genes which distributed in B2-2, B2-3, A0 subgroups, respectively. According to antibiotic susceptibility test, 20 isolates were resistant which belonged to D (D1 phylogenetic subgroup) and A (A0 phylogenetic subgroup) groups.?Conclusion: The results showed that healthy cats could be considered as potential source for the dissemination of ESBL-encoding genes. Further investigations in companion animals and their owners are needed to clarify the importance of spreading of these zoonotic strains.
机译：背景：本研究旨在检测健康猫的粪便样本中产生大范围β-内酰胺酶（ESBLs）的大肠杆菌分离株及其与系统发育背景相关的抗性基因。方法：共50E。大肠杆菌分离物通过标准细菌学测试确认。通过三重PCR方法，将三种遗传标记chuA，yjaA和DNA片段TspE4.C2结合在一起，对分离物进行系统发育分析。检测到ESBL（blaCTXM，blaTEM，blaSHV，blaOXA）编码基因。为了鉴定产生ESBL的表型，对所有选择的菌株进行了双盘协同试验筛选，包括头孢噻肟，头孢噻肟与克拉维酸，头孢他啶和头孢他啶与克拉维酸的结合。结果：结果表明，大肠杆菌分离物分为四个系统发育组（ D，B1和B2）的患病率分别为78％，4％，8％，10％和5个系统发育亚组，包括A0（74％），A1（4％），B1（8％），B2–2（6％） ，分别为B2–3（4％）和D1（4％）。在所有大肠杆菌分离物中，分别分布于B2-2，B2-3，A0亚组的blaSHV，blaCTX-M-15和blaOXA-1基因呈阳性。根据抗生素敏感性试验，有20株耐药菌，分别属于D（D1系统发育亚组）和A（A0系统发育亚组）组。结论：结果表明，健康猫可被视为传播ESBL编码的潜在来源基因。需要进一步研究伴侣动物及其主人，以阐明传播这些人畜共患病菌株的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号