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Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran

机译:伊朗设拉子的金黄色葡萄球菌临床分离株中icaA / icaD基因的检测和生物膜形成

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Introduction: Biofilm producing Staphylococcus aureus is known as one of the majorcausative agents of infections, failure of implanted devices and persistent infectionamong hospitalized patients. The aim of the present study was to determine thefrequency of biofilm producing S. aureus isolates amongst the clinical specimens.Methods: This cross-sectional study was conducted during 2012 to 2013 in twoteaching hospitals in Shiraz, southwest of Iran. Totally, 345 S. aureus isolates fromvarious clinical specimens were included. Biofilm producing isolates werephenotypically detected using Congo Red Agar (CRA) and genotypically by PCRassay for the icaA and icaD genes.Results: Of the 345 S. aureus isolates, 42.3% were methicillin-resistant S. aureus(MRSA) and subsequently 57.7% were methicillin susceptible isolates. The results ofCRA plates showed that 77 (52.7%) and 74 (37.2%) of MRSA and MSSA werebiofilm producing isolates. The frequency of icaA/D genes among MRSA and MSSAisolates was 127 (87%) and 167 (83.9%), respectively.Conclusion: Such a high rate of icaA/D harboring S. aureus among clinical isolatessuggest the risk for establishing persistent infections in the hospital settings.
机译:简介:产生生物膜的金黄色葡萄球菌是引起住院患者感染,植入设备故障和持续感染的主要病因之一。本研究的目的是确定临床标本中产生生物膜的金黄色葡萄球菌分离物的频率。方法:本横断面研究于2012年至2013年在伊朗西南部设拉子的两家教学医院中进行。总共包括来自各种临床标本的345株金黄色葡萄球菌。结果:在345株金黄色葡萄球菌中,耐甲氧西林的金黄色葡萄球菌(MRSA)占42.3%,其次为57.7%。甲氧西林易感菌株。 CRA平板的结果表明,MRSA和MSSA的77种(52.7%)和74种(37.2%)是产生生物膜的分离株。 MRSA和MSSA分离株中icaA / D基因的频率分别为127(87%)和167(83.9%)。医院设置。

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