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首页> 外文期刊>Journal of medical bacteriology. >Detection of tstH Gene in Staphylococcus aureus Isolates from Hospitalized Burnt Children
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Detection of tstH Gene in Staphylococcus aureus Isolates from Hospitalized Burnt Children

机译：住院烧伤儿童金黄色葡萄球菌分离株tstH基因的检测

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Background: The main cause of toxic shock is TSST-1 toxin which is produced by S. aureus. Finding of TSST-1 toxin in burnt children is very important to prevent TSS and its consequences.?Methods: The aim of this study was to investigate the presence of gene encoding TSST-1 toxin in wound specimens by PCR. In this case-control study, 90 children who were admitted to the burn unit, were divided in two groups of 45 patients, namely febrile (cases group) and non-febrile (control group). Samplings were done from the burn wounds and were tested by PCR with specific primers of tstH gene. Finally, all data including demographic characteristics, percentage of burnt surface severity and the PCR results were analyzed, statistically.Results: The positive PCR results indicated the expression of tstH gene in 37.7% of the febrile children and 11.1% of the non-febrile children with a statistically significant difference (p <0.003). The means and the standard deviations for the percentage of burnt surfaces (i.e. severity) in the samples with the positive and negative PCR results were 30.9±16.93 and 20.09±11.02, respectively with a statistically significant difference (p <0.01). No difference with respect to age and sex could be detected between positive and negative PCR results.Conclusion: A direct association between the expression of tstH and the occurrence of fever in the burnt children was observed. Furthermore, increased surface area of the wounds was also positively related to the expression of tstH.
机译：背景：中毒性休克的主要原因是金黄色葡萄球菌产生的TSST-1毒素。在烧伤儿童中发现TSST-1毒素对于预防TSS及其后果非常重要。方法：本研究的目的是通过PCR研究伤口标本中编码TSST-1毒素的基因的存在。在该病例对照研究中，将90例入院烧伤的儿童分为两组，每组45例，即高热（病例组）和非高热（对照组）。从烧伤创面取样，并用tstH基因的特异性引物通过PCR进行测试。最后，统计分析包括人口统计学特征，烧伤表面严重程度百分比和PCR结果在内的所有数据。结果：PCR阳性结果表明tstH基因在37.7％的发热儿童和11.1％的非发热儿童中表达。具有统计学上的显着差异（p <0.003）。 PCR结果为阳性和阴性的样品中烧伤表面百分比（即严重程度）的平均值和标准偏差分别为30.9±16.93和20.09±11.02，差异具有统计学意义（p <0.01）。 PCR阳性和阴性结果在年龄和性别上均无差异。结论：观察到tstH的表达与烧伤儿童发烧直接相关。此外，伤口表面积的增加也与tstH的表达呈正相关。

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