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首页> 外文期刊>Journal of medical bacteriology. >Detection and Molecular Identification of Plasmid Virulence Genes in Salmonella enterica serovar typhimurium Isolated from Human and Animals by Multiplex PCR Method
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Detection and Molecular Identification of Plasmid Virulence Genes in Salmonella enterica serovar typhimurium Isolated from Human and Animals by Multiplex PCR Method

机译：多重PCR法检测人和动物肠炎沙门氏菌鼠伤寒沙门氏菌中毒力基因的检测与分子鉴定

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Background: Salmonella enterica is a zoonotic species that can acquire its resistance in livestock. In humans, Salmonella typhimurium is a major etiological agent of food-borne salmonellosis. The identification of Salmonella spp. by traditional cultural techniques requires 4 to 5 days. The polymerase chain reaction (PCR) offers a simple tool for the rapid detection of Salmonella. Methods: Fifty-five S. typhimurium isolates from bovine, poultry and human sources were isolated and analyzed with biochemical and serological tests. Firstly, multiplex PCR assay with four sets of primers was selected for invA, rfbj, fliC and fljB genes. In the second stage, a simple PCR method with one set primer was applied to detect spvA and spvB genes. Also, multiplex PCR assay with two set primers was carried out to simultaneously detect and identify invA and spvC genes in S. typhimurium. Results: Analysis of the samples showed that while the presence of spvA, spvB and spvC genes in S. typhimurium from the bovine source was 100% (15/15), these same genes were present in 65% (13/20) of the poultry sources. The study also showed that spvA, spvB and spvC genes were present in 85% of human source. Conclusion: This study showed that M- PCR of invA, rfbJ, fljB, and fliC genes were fast, simple, less expensive, accurate and specific in identification S. typhimurium. The advantage of multiplex PCR was that it could simultaneously identify the Salmonella strains which had a virulence plasmid thus facilitating the search for specific etiologic Salmonella serovars. The higher prevalence of spv genes among bovine sources can be injurious for public health.
机译：背景：沙门氏菌是一种人畜共患的物种，可以在牲畜中获得抗药性。在人类中，鼠伤寒沙门氏菌是食源性沙门氏菌病的主要病因。沙门氏菌的鉴定。通过传统文化技巧需要4到5天。聚合酶链反应（PCR）为快速检测沙门氏菌提供了一种简单的工具。方法：从牛，家禽和人源分离出55株鼠伤寒沙门氏菌，并进行生化和血清学分析。首先，针对invA，rfbj，fliC和fljB基因，选择四组引物进行多重PCR分析。在第二阶段，采用一套简单的引物PCR方法检测spvA和spvB基因。另外，用两个引物进行了多重PCR分析，以同时检测和鉴定鼠伤寒沙门氏菌中的invA和spvC基因。结果：对样品的分析表明，虽然来自牛源的鼠伤寒沙门氏菌中存在spvA，spvB和spvC基因为100％（15/15），但这些基因中有65％（13/20）存在相同的基因。家禽来源。研究还表明，在人类来源的85％中存在spvA，spvB和spvC基因。结论：这项研究表明，invA，rfbJ，fljB和fliC基因的M-PCR快速，简单，便宜，准确，特异性高，可用于鉴定鼠伤寒沙门氏菌。多重PCR的优势在于它可以同时鉴定具有毒力质粒的沙门氏菌菌株，从而有助于寻找特定的病原性沙门氏菌血清型。牛来源中spv基因的较高患病率可能危害公共健康。

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