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首页> 外文期刊>Veterinarni Medicina >Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary
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Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary

机译：沙门氏菌亚种中的氯霉素抗性基因。从匈牙利的人类和动物来源分离出的肠型血清鼠伤寒

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摘要

The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar Typhimurium strains isolated from animals and humans in Hungary, by PCR and conjugation. Three groups of chloramphenicol resistant strains were identified. Eleven animal and 13 human isolates harboured the flo gene, encoding resistance to chloramphenicol and florfenicol, and possessed integrons of 1.0 Kb and 1.2 Kb typically found on the multidrug resistance island of S. Typhimurium DT104. Fifteen human strains had two different chloramphenicol resistance genes: the catB3 gene, identified as a gene cassette within a 1.45 Kb integron, and the catA gene, both of which were located on and transferred by a 140 Kb plasmid from a representative strain to the E. coli recipient via conjugation. A single animal strain had the catA gene alone, which was also transferred by a 35 Kb plasmid via conjugation. These three groups of strains belonged to three distinct genetic clusters, as it was revealed by macrorestriction analysis of 18 selected strains. This study provides information on the versatile genetic background of the chloramphenicol and "florfenicol resistances in S. Typhimurium in Hungary and points to the significance of mobile genetic elements such as conjugative R-plasmids and integrons in the spread and persistence of chloramphenicol resistance genes. Theresults also indicate the predominance of the flo gene among animal strains and its appearance among human strains in Hungary.
机译：在匈牙利从动物和人类中分离出的40株肠炎沙门氏菌血清型鼠伤寒沙门氏菌中，研究了氯霉素抗性基因cat，cmlA，flo的存在，以及质粒和1类整合素在氯霉素抗性传播和持久性中的作用，通过PCR和结合。确定了三组氯霉素抗性菌株。 11个动物和13个人类分离株带有flo基因，编码对氯霉素和氟苯尼考的​​抗性，并具有1.0 Kb和1.2 Kb的整合素，通常在鼠伤寒沙门氏菌DT104的多药耐药岛上发现。 15株人类菌株具有两种不同的氯霉素抗性基因：catB3基因（被鉴定为1.45 Kb整合子中的基因盒）和catA基因，两者均位于140 Kb质粒上并通过140 Kb质粒从代表性菌株转移到E大肠杆菌受体通过结合。单个动物品系仅具有catA基因，也通过结合作用由35 Kb质粒转移。这三组菌株属于三个不同的遗传簇，这是通过对18个选定菌株的宏观限制性分析揭示的。这项研究提供了有关氯霉素的通用遗传背景和匈牙利鼠伤寒沙门氏菌中的氟苯尼考耐药性的信息，并指出了移动性遗传元件（如共轭R质粒和整合素）在氯霉素抗性基因的传播和持久性中的重要意义。也表明在匈牙利，flo基因在动物品系中占主导地位，并在人类品系中出现。

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