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首页> 外文期刊>Journal of Medical Biochemistry >Effect of Blood Cell Subtypes Lysis on Routine Biochemical Tests
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Effect of Blood Cell Subtypes Lysis on Routine Biochemical Tests

机译：血细胞亚型裂解对常规生化检验的影响

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Summary Background The aim of this study is to establish the contribution of blood cells subtypes on hemolysis. Methods Separated blood cell subtype suspensions prepared with blood from 10 volunteers were serially diluted to obtain different concentrations of cell suspensions. The cells were fully lysed and cell hemolysates were added (1:20) to aliquots of serum pool. Thus, seven serum pools with different concentrations of interferent were obtained for each blood cell subtype. Biochemical parameters and serum indices were measured by an autoanalyzer. As cell lysis markers, free hemoglobin was measured by spectrophotometry while myeloperoxidase and ?-thromboglobulin were measured by enzyme immunoassay. The percent changes in analyte levels of the serum pools were evaulated by Wilcoxon Signed Rank Test and compared with clinical thresholds defined for each test. Results The clinical thresholds were exceeded in lactate dehydrogenase, potassium, aspartate aminotransferase, creatine kinase, magnesium, total protein, total cholesterol, inorganic phosphate, glucose for red blood cells (RBC); lactate dehydrogenase, aspartate aminotransferase, total protein, inorganic phosphate and glucose for platelets (PLT). Free hemoglobin was significantly correlated with RBC (r=0.999; p=0.001), while myeloperoxidase and b thromboglobulin showed no significant correlation to white blood cells (WBC) and PLT, respectively. Conclusions The effect of RBC hemolysis in serum on the routine biochemical tests are clearly established, yet, additional studies are required in order to verify this kind of effects of PLT and WBC hemolysis.
机译：发明背景本研究的目的是确定血细胞亚型对溶血的贡献。方法连续稀释10名志愿者的血液制备的分离的血细胞亚型悬浮液，以获得不同浓度的细胞悬浮液。将细胞完全溶解，并将细胞溶血产物添加至（1:20）等分的血清池中。因此，对于每种血细胞亚型，获得了七个具有不同干扰素浓度的血清库。通过自动分析仪测量生化参数和血清指标。作为细胞裂解标记物，通过分光光度法测量游离血红蛋白，而通过酶免疫法测量髓过氧化物酶和β-血球蛋白。通过Wilcoxon Signed Rank Test评估血清库中分析物水平的变化百分比，并将其与为每个测试定义的临床阈值进行比较。结果超过了乳酸脱氢酶，钾，天冬氨酸转氨酶，肌酸激酶，镁，总蛋白，总胆固醇，无机磷酸盐，红细胞葡萄糖的临床阈值;乳酸脱氢酶，天冬氨酸转氨酶，总蛋白，无机磷酸盐和血小板葡萄糖（PLT）。游离血红蛋白与RBC显着相关（r = 0.999; p = 0.001），而髓过氧化物酶和b血红蛋白分别与白细胞（WBC）和PLT无显着相关。结论血清RBC溶血对常规生化检查的作用已明确确立，但还需要进一步研究以证实PLT和WBC溶血的这种作用。

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