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首页> 外文期刊>Journal of medical bacteriology. >Molecular Typing of Nocardia Species
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Molecular Typing of Nocardia Species

机译：诺卡氏菌物种的分子分型

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Identification of clinically significant Nocardia species is essential for the definitive diagnosis, predict antimicrobial susceptibility, epidemiological purposes, and for an effective treatment. Conventional identification of Nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic profiles, and chemotaxonomic characteristics is often laborious, and time- consuming. The procedure requires expertise, and newer species can be difficult to differentiate with accuracy from other related species. Alternative methods of identification, such as high performance liquid chromatography (HPLC) and molecular biology techniques allow a better characterization of species. The taxonomy of the genus Nocardia has been dramatically been revised during the last decade and more than 30 valid human clinical significance species of Nocardia have been reported. The use of molecular approaches, including 16S rRNA gene sequencing, restriction fragment length polymorphism (RFLP) or PCR restriction endonuclease analysis has been the focus of recent investigations to distinguish the isolates of Nocardia from other actinomycetes genera. The methods have revolutionized the characterization of the Nocardiae by providing rapid, sensitive, and accurate identification procedures. The present review describes the currently known medically important pathogenic species of Nocardia.
机译：鉴定具有临床意义的诺卡氏菌物种对于明确诊断，预测抗菌药敏感性，流行病学目的和有效治疗至关重要。基于表型（细胞形态，殖民地特征），生化和酶学特征以及化学分类学特征的常规医学实验室中诺卡氏菌的常规鉴定通常是费力且费时的。该程序需要专业知识，较新的物种可能很难与其他相关物种准确区分。其他识别方法，例如高效液相色谱（HPLC）和分子生物学技术，可以对物种进行更好的表征。在过去的十年中，诺卡氏菌属的分类学得到了显着的修订，并且已报告了三十多种诺卡氏菌的有效人类临床意义物种。分子生物学方法的使用，包括16S rRNA基因测序，限制性片段长度多态性（RFLP）或PCR限制性核酸内切酶分析，已成为近期研究中区分诺卡氏菌与其他放线菌属的研究重点。该方法通过提供快速，灵敏和准确的识别程序，彻底改变了诺卡氏菌的特征。本综述描述了诺卡氏菌的当前已知的医学上重要的致病物种。

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