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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of Multiple Differing Copies of the 16S rRNA Gene in Five Clinical Isolates and Three Type Strains of Nocardia Species and Implications for Species Assignment
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Analysis of Multiple Differing Copies of the 16S rRNA Gene in Five Clinical Isolates and Three Type Strains of Nocardia Species and Implications for Species Assignment

机译：诺卡氏菌属的五个临床分离株和三种类型菌株中16S rRNA基因的多个不同拷贝分析及物种分配的意义

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摘要

Five clinical isolates of Nocardia that showed ambiguous bases within the variable region of the 16S rRNA gene sequence were evaluated for the presence of multiple copies of this gene. The type strains of three Nocardia species, Nocardia concava, Nocardia ignorata, and Nocardia yamanashiensis, which also showed ambiguous bases in the variable region, were also examined. Cloning experiments using an amplified region of the 16S rRNA that contains the variable region showed that each isolate possessed 16S rRNA genes with at least two different sequences. In addition, hybridization studies using a 16S rRNA gene-specific probe and extracted genomic DNA of the patient isolates and of the type strain of N. ignorata showed that each isolate possessed at least three copies of the gene. These multiple differing copies of the 16S rRNA gene and the results of DNA-DNA hybridization studies indicate problems of species definition and identification for such isolates. A broader species concept than that currently in vogue may be required to accommodate such organisms.
机译：评估了在16S rRNA基因序列可变区内显示碱基不明确的5种 Nocardia 临床分离株，该基因是否存在多个拷贝。三种诺卡氏菌，诺卡氏菌，诺卡氏菌和山梨诺卡氏菌的三种类型菌株也表现出歧义还检查了可变区中的碱基。使用包含可变区的16S rRNA的扩增区域进行的克隆实验表明，每个分离物均具有带有至少两个不同序列的16S rRNA基因。另外，使用16S rRNA基因特异性探针和患者分离株以及 N型菌株的提取基因组DNA进行杂交研究。 ignorata 表明，每个分离株至少具有该基因的三个拷贝。 16S rRNA基因的这些多个不同拷贝以及DNA-DNA杂交研究的结果表明，此类分离物的物种定义和鉴定存在问题。为了适应这种生物，可能需要比目前流行的物种概念更广泛的概念。

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