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首页> 外文期刊>Journal of Medical Sciences >Assessing Mutagenicity of Methanolic Exteract of Abrus precatorius Seeds using Ames Bioassay
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Assessing Mutagenicity of Methanolic Exteract of Abrus precatorius Seeds using Ames Bioassay

机译：使用Ames生物测定法评估普鲁氏鲍鱼种子甲醇提取物的致突变性

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Abrus precatorius seeds contain toxin abrin which inhibits protein synthesis and leading to cell death. This plant has Cytotoxic and pro-apoptotic effects. So, in this study, mutagnicity of methanolic seed extract of this plant was investigated using Ames test. The seeds were powdered and then maceration extracted with methanol for 48 h. Extract was analyzed for aflatoxin B1 and histidine contamination with TLC and MIC determined by broth dilution method. The test was performed using strain TA100. The genotypes was confirmed by presence of hisG46 Mutation, R factor plasmid and crystal violet sensitivity test.TA100 carries mutations in genes involved in histidine synthesis, so that it requires histidine for growth. Tests were performed with 4 concentrations 0.2, 0.3, 0.4 and 0.5 mg mL-1 (concentrations less than 0.1 MIC), negative control (methanol), positive control (NaN3), in the absence and presence of a rat liver metabolizing system. No increase in bacteria growth in the histidine-free medium was observed. In conclusion, Abrus precatorius was not genotoxic in Ames test. However, it is likely that the Abrus precatorius maybe inducing gonotoxicity at high concentrations. Thus, it is necessary to pay close attention while utilizing its products.
机译：早熟小麦种子含有毒素蛋白，抑制蛋白质合成并导致细胞死亡。该植物具有细胞毒性和促凋亡作用。因此，在这项研究中，使用Ames试验研究了该植物的甲醇种子提取物的致突变性。将种子粉化，然后用甲醇浸软浸提48小时。用肉汤稀释法通过TLC和MIC分析提取物中的黄曲霉毒素B1和组氨酸污染。使用TA 100 菌株进行测试。通过HisG46突变，R因子质粒的存在和结晶紫敏感性试验证实了该基因型。TA 100 在参与组氨酸合成的基因中携带突变，因此需要组氨酸才能生长。用4种浓度的0.2、0.3、0.4和0.5 mg mL -1 （浓度小于0.1 MIC），阴性对照（甲醇），阳性对照（NaN 3 ）进行测试），不存在大鼠肝脏代谢系统。在无组氨酸的培养基中未观察到细菌生长的增加。总之，Abrus precatorius在Ames试验中没有遗传毒性。但是，很可能Abrus precatorius可能在高浓度下诱发淋病。因此，在使用其产品时必须密切注意。

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