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首页> 外文期刊>Journal of Medical Sciences >Phytochemical, Toxicity, Antimicrobial and antioxidant Screening of Extracts Obtained from Laportea aestuans (Gaud)
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Phytochemical, Toxicity, Antimicrobial and antioxidant Screening of Extracts Obtained from Laportea aestuans (Gaud)

机译：东方香茅提取物的植物化学，毒性，抗菌和抗氧化剂筛选

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Phytochemicals responsible for toxicity, antimicrobial and antioxidant activities of extracts obtained from Laportea aestuans (Gaud) were investigated. Secondary metabolites detected were alkaloids, tannins, resins, saponins and carbohydrate. Flavonoids, sterols, cardiac glycosides, phenols, glycosides were however beyond detectable limit. Brine shrimp lethality test on the partition fractions revealed that hexane, ethyl acetate and butanol fractions were toxic with a lethal dose (LC50) less than 1000 μ mL-1 while the crude methanol extract with LC50 greater than 1000 μ mL-1 was non-toxic. The antimicrobial assay of the crude extract and their fractions were carried out by agar well diffusion and pour plate methods against 6 bacteria ( Staphylococcus aureus , Escherichia coli , Bacillus subtilis , Pseudomonas aeruginosa , Klebsiellae pneumonae , Salmonella typhi ) and 4 fungi ( Candida albicans , Aspergillus niger , Rhizopus stolon , Penicillum notatum ). All the extracts had broad spectrum antimicrobial effect at the various concentrations when compared with gentamicin and tioconazole (antibacterial and antifungal standards respectively). The antioxidant activity of L. aestuans was determined by three methods; scavenging effect on 2,2-diphenyl-1-picryhydrazyl radical (DPPH), hydroxyl radical scavenging effect and ferric thiocynate methods and it was revealed the fractions possessed significant antioxidant activity when compared with antioxidant standards butylated hydroxyl anisole (BHA), ascorbic acid and α-tocopherol used in the assay.
机译：研究了从Laportea aestuans（Gaud）获得的提取物的毒性，抗菌和抗氧化活性的植物化学物质。检测到的次要代谢产物是生物碱，单宁，树脂，皂苷和碳水化合物。然而，类黄酮，固醇，强心苷，酚，糖苷的含量超出了可检测的范围。盐水虾对分配级分的致死性测试表明，己烷，乙酸乙酯和丁醇级分具有毒性，其致死剂量（LC 50 ）小于1000μmL -1 ，而LC 50 大于1000μmL -1 的粗甲醇提取物无毒。通过琼脂井扩散和倾盘法对6种细菌（金黄色葡萄球菌，大肠杆菌，枯草芽孢杆菌，铜绿假单胞菌，肺炎克雷伯菌，伤寒沙门氏菌）和4种真菌（白色念珠菌，黑曲霉，根霉，青霉）。与庆大霉素和噻康唑（分别为抗菌和抗真菌标准品）相比，所有提取物在不同浓度下均具有广谱抗菌作用。三种方法测定了通心草的抗氧化活性。对2,2-二苯基-1-吡啶并肼基（DPPH）的清除作用，对羟基的清除作用和硫代铁酸化方法的结果表明，与抗氧化剂标准丁基化羟基茴香醚（BHA），抗坏血酸和分析中使用的α-生育酚。

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