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首页> 外文期刊>Journal of Medical Sciences >Altered Proliferative Response of Normal and Malignant Cells Exposed to a Polysaccharide Extract from Tamarindus indica Pulp
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Altered Proliferative Response of Normal and Malignant Cells Exposed to a Polysaccharide Extract from Tamarindus indica Pulp

机译：罗望子果肉多糖提取物对正常和恶性细胞增殖反应的改变

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摘要

A water-soluble Polysaccharide Extract (PE) isolated from the pulp of the fruit bodies of a Mexican species of Tamarindus indica L. was characterized by thin-layer chromatography and HPLC. Fructose, glucose, arabinose and xylose were the major components of the hydrolyzed PE samples. Normal lymphocytes and several lineages of malignant cells were cultured in presence of different amounts of PE solution and their effects on cellular proliferation were assessed by tritiated thymidine uptake. After incubation for 48 h, a significant reduction on the thymidine uptake was observed when Con-A-stimulated mice?s splenocytes were incubated with tamarind PE. Furthermore, a similar reduction in proliferative activity was detected also in CTLL-2 and HeLa cell lines. A comparative control assay showed a more intense inhibition when similar amounts (5-100 μg mL-1) of cis-platinum was added to HeLa cells. The morphological examination of PE-treated HeLa cells revealed their transformation into multinucleated giant cells. The results suggest that polysaccharide extracts obtained from tamarind pulp may contain active inhibitors of in vitro cellular multiplication.
机译：从墨西哥罗望子属墨西哥种的子实体果肉中分离得到的水溶性多糖提取物（PE）用薄层色谱和HPLC进行了表征。果糖，葡萄糖，阿拉伯糖和木糖是水解PE样品的主要成分。在不同量的PE溶液中培养正常淋巴细胞和一些恶性细胞系，并通过tri化胸腺嘧啶核苷的摄取评估它们对细胞增殖的影响。孵育48小时后，当用罗望子PE孵育Con-A刺激的小鼠脾细胞时，胸腺嘧啶核苷的摄取显着降低。此外，在CTLL-2和HeLa细胞系中也检测到了类似的增殖活性降低。当向HeLa细胞中添加相似量的（5-100μgmL -1 ）顺铂时，对比对照试验显示出更强的抑制作用。经PE处理的HeLa细胞的形态学检查显示它们已转化为多核巨细胞。结果表明，从罗望子果肉获得的多糖提取物可能含有体外细胞增殖的活性抑制剂。

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