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首页> 外文期刊>Journal of Medicinal Plants Research >Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts
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Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts

机译：提取工艺对孟库杜叶提取物中酚类成分和抗氧化活性的影响

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摘要

The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE)?on the phenolic components and antioxidant activity of?Morinda citrifolia?leaf extracts was investigated.?Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method, while the catechins were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). Antioxidant activity of the extracts was evaluated by determining 2,2-diphenyl-1-picrylhydrazyl (DPPH) capacity and ferric ion reducing antioxidant potential (FRAP). Overall, MAE produced extract exhibited the maximum amount of TPC and catechin while UAE-extract had the highest antioxidant activity. It could be concluded that, even though MAE extract contained high TPC and catechin, the accelerated temperature used during this extraction technique might have attributed to reduce the antioxidant activity of this extract. Therefore, UAE can be recommended for recovery of potent natural antioxidant components from?M. citrifolia?leaf offering better antioxidant activity.
机译：四种提取技术的效果分别是：考察了溶剂萃取（SE），微波辅助萃取（MAE），超声辅助萃取（UAE）和超临界流体萃取（SFE）对柑桔叶提取物的酚类成分和抗氧化活性的影响。 （TPC）使用Follin-Ciocalteu试剂法分光光度法定量，儿茶素通过反相高效液相色谱（RP-HPLC）分析。提取物的抗氧化活性通过确定2，2-二苯基-1-吡啶并肼基（DPPH）容量和还原铁离子的抗氧化电位（FRAP）进行评估。总的来说，MAE生产的提取物表现出最大的TPC和儿茶素含量，而阿联酋提取物具有最高的抗氧化活性。可以得出结论，即使MAE提取物含有较高的TPC和儿茶素，在此提取技术中使用的加速温度可能归因于降低了该提取物的抗氧化活性。因此，建议使用阿联酋从？M中回收有效的天然抗氧化剂成分。柠檬叶提供更好的抗氧化活性。

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