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首页> 外文期刊>Journal of medical bacteriology. >Assessment of AmpC Beta-Lactamase Genes among Clinical Escherichia coli Isolates
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Assessment of AmpC Beta-Lactamase Genes among Clinical Escherichia coli Isolates

机译：临床大肠杆菌分离株中AmpCβ-内酰胺酶基因的评估

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Background: AmpC beta lactamases play a significant role in creating resistance to third generation cephalosporins worldwide. They mostly express on chromosome of Enterobacteriaceae especially Escherichia coli and cause consequential problem in clinical treatment and lead to failure in diagnosis and phenotypic test recommended by Clinical and Laboratory Standards Institute.?Methods: Totally 200 E. coli isolates from different hospitals of Tehran were collected. The isolates were screened by disk diffusion method according to the CLSI guidelines. The profiles and prevalence surveys of AmpC (Dha, CITM, Mox and FOX-type) β-lactamase genes in clinical isolates of E. coli by phenotypic and molecular methods.?Results: Out of 200 E. coli isolated, 115 (89.8%) and 13 (10.2%) isolates were identified as ESBL- and AmpC- beta-lactamase producers, respectively. Among AmpC producers, 13 (100%) and 5 (38.5%) isolates was reported by PCR assay as blaCITM and Dha, respectively. Mox and FOX genes were not detected in any sample.Conclusion: Our results highlight the importance of using molecular detection methods to identify β-lactamase-producer that have resistance to antibiotics.
机译：背景：AmpCβ内酰胺酶在全球范围内对第三代头孢菌素产生耐药性中起着重要作用。它们主要在肠杆菌科特别是大肠杆菌的染色体上表达，并在临床治疗中引起相应的问题，并导致临床和实验室标准协会推荐的诊断和表型测试失败。方法：收集来自德黑兰不同医院的200株大肠杆菌分离株。 。根据CLSI指南，通过盘扩散法筛选分离物。通过表型和分子方法对大肠杆菌临床分离株中AmpC（Dha，CITM，Mox和FOX型）β-内酰胺酶基因进行了概况和流行情况调查。结果：在分离出的200株大肠杆菌中，有115株（占89.8％） ）和13（10.2％）分离株分别被鉴定为ESBL-和AmpC-β-内酰胺酶生产者。在AmpC生产商中，通过PCR测定报告了13种（100％）和5种（38.5％）分离株，分别为blaCITM和Dha。结论：我们的结果突出了使用分子检测方法鉴定对抗生素具有抗性的β-内酰胺酶生产者的重要性。

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