...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medicinal Plants Research >Pretreatment with astragaloside IV protects H9c2 cells against hydrogen peroxide-induced apoptosis by scavenging of reactive oxygen species and regulation of Bcl-2 and Bax expression
【24h】

Pretreatment with astragaloside IV protects H9c2 cells against hydrogen peroxide-induced apoptosis by scavenging of reactive oxygen species and regulation of Bcl-2 and Bax expression

机译：黄芪甲苷IV预处理通过清除活性氧以及调节Bcl-2和Bax表达来保护H9c2细胞免于过氧化氢诱导的细胞凋亡

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Astragaloside IV (ASI), a saponin isolated from?Radix Astragali, has been found to have potent cardioprotective effects. This study was designed to investigate whether ASI prevents cardiomyocytes from hydrogen peroxide (H2O2)-induced apoptosis. H9c2 cells were pretreated with different concentrations of ASI (5, 10, 20 μg/ml) for 24 h and then exposed to 100 μM H2O2?for 24 h. The cell viability were examined by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide)?assay,?reactive oxygen species(ROS) generation was quantified by the 2',7'-dichlorofluorescin-diacetate (DCFH-DA) method. The activity of antioxidant enzymes, including catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were determined photometrically. Apoptotic cells were detected by Hoechst 33258 staining, annexin-V binding and by assessment of caspase-3. Protein and mRNA expression of both Bax and Bcl-2 were determined by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). The cell apoptosis significantly increased after 24 h of H2O2?exposure. Pretreatment of H9c2 cells with ASI significantly increased the activities of antioxidant enzymes, scavenged ROS and reduced?malondialdehyde (MDA)?production. ASI increased the expression of Bcl-2, decreased the expression of Bax, and ultimately reduced H2O2-induced H9c2 cells apoptosis. In summary, these results suggest that ASI can block H2O2-induced apoptosis in H9c2 cells, and that the underlying mechanism involves in?scavenging of ROS?and modulating expression of Bcl-2 and Bax.
机译：从黄芪中分离出的皂苷黄芪甲苷IV（ASI）被发现具有有效的心脏保护作用。这项研究旨在调查ASI是否能阻止心肌细胞免于过氧化氢（H2O2）诱导的细胞凋亡。用不同浓度的ASI（5、10、20μg/ ml）预处理H9c2细胞24小时，然后将其暴露于100μMH2O2？中24小时。用MTT（3-（4,5-二甲基噻唑-2-基）-2，5-二苯基溴化四溴化铵）？检测法检测细胞活力，？活性氧（ROS）的生成量通过2'，7'-二氯荧光素-二乙酸酯（DCFH-DA）方法。用光度法测定了抗氧化酶的活性，包括过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）。通过Hoechst 33258染色，膜联蛋白-V结合和通过评估caspase-3来检测凋亡细胞。通过蛋白质印迹和逆转录-聚合酶链反应（RT-PCR）测定Bax和Bcl-2的蛋白质和mRNA表达。 H2O2暴露24 h后，细胞凋亡明显增加。用ASI预处理H9c2细胞可显着增加抗氧化酶的活性，清除ROS和减少丙二醛（MDA）的产生。 ASI增加Bcl-2的表达，降低Bax的表达，并最终减少H2O2诱导的H9c2细胞凋亡。总之，这些结果表明，ASI可以阻断H2O2诱导的H9c2细胞凋亡，其潜在机制涉及清除ROS和调节Bcl-2和Bax的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号