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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >CHARACTERISTIC OF STREPTOMYCES SPECIES WITH ANTIMICROBIAL ACTIVITY AGAINST SELECTED PHYTOPATHOGENIC BACTERIA AND FUNGI
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CHARACTERISTIC OF STREPTOMYCES SPECIES WITH ANTIMICROBIAL ACTIVITY AGAINST SELECTED PHYTOPATHOGENIC BACTERIA AND FUNGI

机译：链霉菌属物种对某些致病菌和真菌的抗菌活性

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The object of this study was to find and characterise streptomycete isolates with antimicrobial activity accomplished by the agar plug method against phytopathogenic species. The test-microorganisms were selected according to their importance in agriculture. All of them belong to phytopathogenic species which reduce yields of agriculturally important plants worldwide. A total number of four phytopathogenic bacteria (Xanthomonas campestris (CCM 22), Pseudomonas syringae (CCM 2868), Erwinia amylovora (CCM 1114), Clavibacter michiganensis subsp. sepedonicus (CCM 7014)) and four phytopathogenic fungi (Botrytis cinerea, Fusarium poae, Alternaria tenuissima, Alternaria arborescens) were used for this experiment. Overall twenty streptomycete isolates showed antimicrobial activity against at least two of the test-microorganisms. These active isolates were subsequently characterized. Streptomycete isolates were observed for morphological characteristics, such as morphology and colour of aerial and substrate mycelium, colour of diffusible pigments, production of melanoid pigments on peptone-yeast extract iron agar and sporophore and spore chain morphology following the International Streptomyces Project. Genes coding polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS), which are considered to responsible for the synthesis of large number of biologically active compounds, like antibiotics and antifungals, that are produced by Streptomyces species largely, were screened. The occurrence of these genes in the genome of our isolates was in accordance with results from antimicrobial activity analysis. Classification to genus Streptomyces were confirmed by DNA sequencing.
机译：这项研究的目的是发现和鉴定具有琼脂塞入法对植物病原菌具有抗菌活性的链霉菌分离物。根据测试微生物在农业中的重要性来选择。它们全部属于植物致病物种，其降低了全世界农业重要植物的产量。总共有四种植物病原菌（黄单胞菌（CCM 22），丁香假单胞菌（CCM 2868），解淀粉欧文氏杆菌（CCM 1114），密闭克氏杆菌亚种（CCM 7014））和四种植物病原性真菌（灰霉病，灰霉病该实验使用了链格孢菌（Alternaria tenuissima）。总体上，二十种链霉菌分离物显示出对至少两种测试微生物的抗菌活性。随后表征了这些活性分离物。根据国际链霉菌项目，观察到链霉菌分离物的形态特征，例如气态和底物菌丝体的形态和颜色，可扩散色素的颜色，蛋白one-酵母提取铁琼脂和孢子体上的类黑素色素和孢子和孢子链的形态。筛选了编码聚酮化合物合酶（PKS-1）和非核糖体肽合成酶（NRPS）的基因，这些基因被认为负责合成大量由链霉菌属物种产生的生物活性化合物，例如抗生素和抗真菌药。这些基因在我们分离株的基因组中的出现与抗菌活性分析的结果一致。通过DNA测序证实链霉菌属的分类。

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