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Isolation and antimicrobial activity of Streptomyces tubercidicus against pathogenic bacteria and fungi

机译:链霉菌对致病性细菌和真菌的分离和抗微生物活性

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Streptomyces spp. produces many secondary metabolites, including antibiotics. The substances formation is coupled with the onset of development of the microorganism and the search of new substances is important. The aim of this work was to evaluate the bioactivity of endophytic Streptomyces tubercidicus isolated from Solanum lycocarpum St. Hill, a typical Brazilian tropical savannah tree to test its inhibitory capability against pathogenic bacteria and fungi. S. tubercidicus was cultivated in ISP2A and submitted to antibiosis test against S. aureus (ATCC 29213), P. aeruginosa (ATCC 27853), E. coli (ATCC 25922) and C. albicans (ATCC 10231). S. tubercidicus presented antibiosis against S. aureus (35 mm), E. coli (40 mm) and C. albicans (19 mm), but failed to inhibit P. aeruginosa. Purification of crude extract of S. tubercidicus with permeation in Sephadex L20 gel methanol revealed two compounds with bioactivity against S. aureus and E. coli.
机译:Streptomyces spp。产生许多次级代谢物,包括抗生素。该物质形成与微生物发育的开始,并对新物质进行搜索是重要的。这项工作的目的是评估内生心肌霉菌的生物活性来自Solanum Lycocarpum St. Hill,典型的巴西热带大草原树,以测试其针对致病细菌和真菌的抑制能力。 S. Tubercidicus在ISP2A中培养,并针对针对金黄色葡萄球菌(ATCC 29213),P.铜绿假单胞菌(ATCC 27853),大肠杆菌(ATCC 25922)和C. albicans(ATCC 10231)的抗分炎试验。 S. Tubercidicus呈现针对金黄色葡萄球菌(35mm),大肠杆菌(40 mm)和C. albicans(19 mm)的抗抗体,但未抑制P.铜绿假单胞菌。 Sephadex L20凝胶甲醇中渗透渗透渗出的粗提物的纯化揭示了两种具有对金黄色葡萄球菌和大肠杆菌的生物活性的化合物。

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