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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >CLONING AND EXPRESSION MOST EXPECTED ANTIGENIC FRAGMENT OF BETA-TOXIN GENE FROM CLOSTRIDIUM PERFRINGENS TYPE B
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CLONING AND EXPRESSION MOST EXPECTED ANTIGENIC FRAGMENT OF BETA-TOXIN GENE FROM CLOSTRIDIUM PERFRINGENS TYPE B

机译：B型产气荚膜梭菌β-毒素基因的克隆和表达预期的抗原性片段

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摘要

Clostridium perfringens type B and C is an important pathogen and produces Beta-toxin which are responsible necrotic enteritis in humans or livestock. The death in individuals with this disease are over 50%. Vaccines against C. perfringens type B and C are currently manufactured using Beta-toxin produced by the virulent C. perfringens strain itself. To achieve the effective components for the creation of immunity ?at the first step used different primers in various location of Beta-toxin gene (cbp) by bioinformatics tools according to the secondary protein structure. After amplication of PCR products, one regions of Beta-toxin gene with high antigenicity was cloned into pTZ57RT and sub-cloned into the expression vector pET21a??(+). The cloned vector was transformed into E. coli BL21 (DE3) and successfully expressed. Protein expression was confirmed by SDS-PAGE electrophoresis and western blotting. This recombinant peptide from most antigenic region of Beta-toxin gene can be suggested for antibody production and new peptide vaccine.
机译：B型和C型产气荚膜梭菌是一种重要的病原体，会产生Beta毒素，这种毒素是人类或牲畜的坏死性肠炎。患有这种疾病的人的死亡超过50％。目前，B。和C.型产气荚膜梭状芽孢杆菌的疫苗是使用有毒的C.产气荚膜梭菌菌株本身产生的β-毒素生产的。为了获得产生免疫力的有效成分，第一步，根据二级蛋白质结构，通过生物信息学工具在β-毒素基因（cbp）的不同位置使用了不同的引物。 PCR产物扩增后，将具有高抗原性的β-毒素基因的一个区域克隆到pTZ57RT中，再亚克隆到表达载体pET21aβ（+）中。克隆的载体被转化到大肠杆菌BL21（DE3）中并成功表达。通过SDS-PAGE电泳和蛋白质印迹证实蛋白质表达。这种来自β-毒素基因大多数抗原区域的重组肽可用于抗体生产和新型肽疫苗。

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