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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >GENETIC DIVERSITY OF WINTER WHEAT (Triticum aestivum L.)
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GENETIC DIVERSITY OF WINTER WHEAT (Triticum aestivum L.)

机译：冬小麦遗传多样性（Triticum aestivum L.）

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Cereals form an indispensable part of the human diet, and wheat is one of the most important agricultural commodities worldwide. Technological quality of wheat grain is determined mainly by the representation of gluten proteins. The aim of this work was to evaluate the technological quality of winter wheat grain based on genetic markers rested on polymorphism of storage proteins. We analyzed ten varieties of winter wheat (Triticum aestivum L.), in which we determined the content of crude proteins and fractional composition of proteins. Then we analyzed the samples by electrophoretic methods using SDS – PAGE and A – PAGE protocols, which allowed us to separate storage proteins and subsequently to detect the presence of individual HMW-GS as molecular markers of breadmaking quality of wheat. We calculated Glu-score based on the representation of HMW-GS. According to SDS – PAGE, the most common composition of HMW-GS was formed by alleles 0, 7+9, 5+10, which occurred in 30 % of analyzed varieties (Natanael, Silvanus, Genoveva). The highest Glu-score (10) was achieved by the variety Ilias. From fingerprinting of gliadins in A - PAGE we constructed a dendrogram of relatedness of individual analyzed varieties. In dendrogram, most genetically distant varieties according to gliadins polymorphism are the varieties Silvanus from the varieties Bardotka, Sophytra, Faustina and Genoveva; also Natanael from the variety Sophytra. Genetically closest from this point of view is the variety Federer to the variety Panonnicus, as well as Natanael to Silvanus.
机译：谷物是人类饮食中不可或缺的一部分，而小麦是全世界最重要的农产品之一。小麦籽粒的技术品质主要取决于面筋蛋白的含量。这项工作的目的是基于基于存储蛋白多态性的遗传标记来评估冬小麦籽粒的技术质量。我们分析了十个冬小麦品种（Triticum aestivum L.），在其中确定了粗蛋白的含量和蛋白的分数组成。然后，我们使用SDS – PAGE和A – PAGE方案通过电泳方法分析了样品，这使我们能够分离存储蛋白，并随后检测单个HMW-GS的存在，以此作为小麦面包品质的分子标记。我们基于HMW-GS的表示来计算Glu得分。根据SDS – PAGE，HMW-GS的最常见组成是由等位基因0、7 + 9、5 + 10形成，这些等位基因出现在30％的分析品种中（纳塔内尔，西尔瓦努斯，热诺娃）。品种Ilias的Glu得分最高（10）。根据A-PAGE中麦醇溶蛋白的指纹图谱，我们构建了各个分析品种相关性的树状图。在树状图中，根据麦醇溶蛋白多态性的遗传上最远的品种是来自Bardotka，Sophytra，Faustina和Genoveva品种的Silvanus品种。还有Sophytra品种的Natanael。从遗传学角度来看，费德勒（Federer）品种与Panonnicus品种以及Natanael与Silvanus品种在遗传上最接近。

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