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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >CHARACTERIZATION OF A BOSEA SP. STRAIN SF5 (MTCC 10045) ISOLATED FROM COMPOST SOIL CAPABLE OF PRODUCING CELLULASE
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CHARACTERIZATION OF A BOSEA SP. STRAIN SF5 (MTCC 10045) ISOLATED FROM COMPOST SOIL CAPABLE OF PRODUCING CELLULASE

机译：BOSEA SP的特性。 SF5（MTCC 10045）与产生纤维素酶的堆肥土壤隔离

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A cellulase producing bacterium, designated SF5 was isolated from compost soil. The strain was identified as Bosea sp. based on 16S rRNA gene sequence analysis and phenotypic characters including detail carbon sources utilization pattern. The effect of various carbohydrates such as Carboxy Methyl Cellulose (CMC) avicel, starch, maltose, sucrose, glucose, fructose and lactose (as carbon source) on cellulase production revealed that 0.75% CMC (with 8 days incubation) was optimum. Among the various nitrogen sources, 0.15% NH4Cl gave optimal production of cellulase. The optimal conditions for the production of cellulase by strain SF5 were determined to be at 37 oC temperature and at pH 7.0. The strain is also capable of producing xylanase and may have biotechnological potential.
机译：从堆肥土壤中分离出一种产生纤维素酶的细菌，命名为SF5。该菌株被鉴定为Bosea sp。基于16S rRNA基因序列分析和表型特征，包括详细的碳源利用模式。各种碳水化合物（例如羧甲基纤维素（CMC）avicel，淀粉，麦芽糖，蔗糖，葡萄糖，果糖和乳糖（作为碳源））对纤维素酶生产的影响表明，0.75％CMC（孵育8天）是最佳的。在各种氮源中，0.15％NH4Cl可使纤维素酶产生最佳产量。确定菌株SF5产生纤维素酶的最佳条件是在37 oC温度和pH 7.0下。该菌株还能够产生木聚糖酶，并且可能具有生物技术潜力。

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