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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >IN VITRO STUDY OF THE EFFECT OF 17β-ESTRADIOL AND 4-NONYLPHENOL ON BOVINE SPERMATOZOA
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IN VITRO STUDY OF THE EFFECT OF 17β-ESTRADIOL AND 4-NONYLPHENOL ON BOVINE SPERMATOZOA

机译：17β-雌二醇和4-壬酚对牛精子的影响的体外研究

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Nonylphenol (NP), an environmental endocrine disruptor, is a final metabolite of nonylphenol ethoxylate (NPE) that is able to interfere with hormonal system of numerous organisms. Estrogens play a central role in female reproduction, but also affect the male reproductive system. In males, stimulate mammalian spermatozoa capacitation, acrosome reaction and fertilizing ability. The aim of this in vitro study was to determine the effect of 17β-estradiol and nonylphenol (NP) on the spermatozoa motility. Specifically, we examined the dose- and time-dependent effect of nonylphenol (1, 10, 100 and 200 μg/mL) with the addition 1 μg/mL of 17β-estradiol on the motility and progressive motility of bovine spermatozoa during two time periods (0 h and 6 h). The spermatozoa motility was determined by CASA (Computer Assisted Semen Analyzer) system using the Sperm VisionTM program. The results showed decreased average values of motility in all experimental groups during 0 h of in vitro cultivation. The motility and the progressive motility of bovine spermatozoa increased in the experimental groups using concentrations 10, 100 and 200 μg/mL after 6 h of cultivation and significant differences (P<0.05) were detected between these groups and the control group. The results suggest that the addition of 17β-estradiol could positively affect spermatozoa motility during the short-term cultivation of spermatozoa with NP.
机译：壬基酚（NP）是一种环境内分泌干扰物，是壬基酚乙氧基化物（NPE）的最终代谢产物，能够干扰多种生物的激素系统。雌激素在女性生殖中起着核心作用，但也会影响男性生殖系统。在雄性中，刺激哺乳动物的精子获能，顶体反应和受精能力。这项体外研究的目的是确定17β-雌二醇和壬基酚（NP）对精子活力的影响。具体来说，我们研究了壬基酚（1、10、100和200μg/ mL）与1μg/ mL17β-雌二醇的添加在两个时间段内对牛精子运动性和进行性运动的剂量和时间依赖性作用（0小时和6小时）。精子活力是通过CASA（计算机辅助精液分析仪）系统使用Sperm VisionTM程序测定的。结果表明，在体外培养0 h期间，所有实验组的平均运动力均降低。培养6 h后，使用浓度分别为10、100和200μg/ mL的实验组，牛精子的活力和进行性活力增加，并且在这些组和对照组之间存在显着差异（P <0.05）。结果表明，在NP短期培养精子过程中，添加17β-雌二醇可对精子活力产生积极影响。

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