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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >PRODUCTION AND PHYSICOCHEMICAL PROPERTIES OF THERMOSTABLE, CRUDE CELLULASE FROM ENTEROBACTER CLOACAE IP8 ISOLATED FROM PLANT LEAF LITTERS OF LAGERSTROEMIA INDICA Linn.
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PRODUCTION AND PHYSICOCHEMICAL PROPERTIES OF THERMOSTABLE, CRUDE CELLULASE FROM ENTEROBACTER CLOACAE IP8 ISOLATED FROM PLANT LEAF LITTERS OF LAGERSTROEMIA INDICA Linn.

机译：产自印度紫薇植物叶片的肠球菌IP8的热稳定粗纤维素酶的生产和理化性质。

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摘要

Cellulases have wide applications and biotechnological potentials for various industries. A bacterium producing an extracellular, thermostable cellulase was isolated from plant leaf litters of Lagerstroemia indica Linn, inside a botanical garden. According to morphological, biochemical and physiological characterization, it was tentatively identified as Enterobacter sp. Molecular characterization, using the 16S rRNA gene sequencing was used to confirm the identity of the bacterium as Enterobacter cloacae IP8. Effects of some cultural factors such as carbon and nitrogen sources, pH and temperature, on cellulase production from the bacterium, were investigated. Some physicochemical properties of the crude cellulase from E. cloacae IP8 were determined to evaluate its potentials for industrial applications. The maximum yield of cellulase (10.78 U/mL) was at 28 h of incubation using carboxymethyl cellulose (CMC) (1.5%, w/v), peptone (2.0%, w/v), inoculum size (1.0%, v/v), pH and temperature of 7.0 and 45 oC, respectively, and agitation speed 150 rpm. The crude cellulase exhibited optimum activity at 60 oC, retaining 75.0% of its maximal activity at 70 oC. It had optimum pH of 7.0, retaining 58.0% of its original activity at acidic pH 5.0. Metal ions Na+, Ca2+ and Mg2+ remarkably enhanced activity of the cellulase while K+ and EDTA inhibited activity of the cellulase from E. cloacae IP8. The characteristics of the cellulase from E. cloacae IP8 revealed the enzyme as being thermostable and an acidic to neutral metalloenzyme. Therefore, the enzyme from this strain could be applied in industrial applications such as lignocellulosic biomass conversion into fuel and other value added products.
机译：纤维素酶在各种行业中具有广泛的应用和生物技术潜力。从植物园内的印度紫薇（Lagerstroemia indica Linn）的植物叶子凋落物中分离出产生细胞外热稳定纤维素酶的细菌。根据形态，生化和生理学特征，初步鉴定为肠杆菌。使用16S rRNA基因测序进行分子鉴定，以确认该细菌为阴沟肠杆菌IP8。研究了一些文化因素，例如碳和氮源，pH和温度对细菌产生纤维素酶的影响。确定了阴沟肠杆菌IP8粗纤维素酶的一些理化性质，以评估其工业应用潜力。在使用羧甲基纤维素（CMC）（1.5％，w / v），蛋白ept（2.0％，w / v），接种量（1.0％，v / v）孵育28小时后，纤维素酶的最大产量（10.78 U / mL）为v），pH和温度分别为7.0和45 oC，搅拌速度为150 rpm。粗纤维素酶在60 oC时显示最佳活性，在70 oC时保留其最大活性的75.0％。它的最佳pH为7.0，在酸性pH 5.0下仍保持其原始活性的58.0％。金属离子Na +，Ca2 +和Mg2 +显着增强了纤维素酶的活性，而K +和EDTA抑制了阴沟肠杆菌IP8产生的纤维素酶的活性。阴沟肠杆菌IP8的纤维素酶特性表明该酶是热稳定的，是酸性至中性的金属酶。因此，来自该菌株的酶可用于工业应用，例如将木质纤维素生物质转化为燃料和其他增值产品。

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