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首页> 外文期刊>Journal of Microbial & Biochemical Technology >Comparison of IAA Production by Shallot Rhizosphere Isolated Bacteria in Solid and Liquid Media and Their Effect on Shallot Plant Growth
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Comparison of IAA Production by Shallot Rhizosphere Isolated Bacteria in Solid and Liquid Media and Their Effect on Shallot Plant Growth

机译：固态和液态介质中青葱根际分离细菌生产IAA的比较及其对青葱植物生长的影响

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Some of free-living rhizobacteria were isolated from rhizosphereof shallot growing in two different fields on Sulawesi Island. The bacterial isolates were cultured in liquid and solid media and were further tested for their ability to produce bioauxin. L-Tryptophan as a physiological precursor of auxin was added to the culture media and the production of IAA was tested using a colorimeter method. Six isolates from west Sulawesi and ten isolates from South Sulawesi were found to produce bioauxin, while 40 other isolates had negative results on IAA production. The amount of IAA produced by the isolates on liquid medium ranged from 4.01 to 8.62 ppm, while in the solid medium, the concentration of IAA produced by the same bacteria was considerably lower than in Liquid media. Thus, culture conditions influence the secretion of IAA by the bacteria. Of these 16 IAA producing isolates, five efficient producers were used for in plant a growth promotion assay. The isolate MK6-1-1 showed the best stimulatory effect on leaves number and bulb tillers, isolate LB8 was investigated to modulate better bulbs fresh biomass weight, while isolate MK11 was found to have the best effect on bulbs dry weight and bulbs dry biomass weight. Application of liquid media MK 6-1-1 bacteria isolates on the medium shallot planting early phases provides the best results on the vegetative growth of shallot that is leafs number (10.75 pieces) and the number of bulb tillers (2.75 bulbs) except the best plants height showed by Isolate bacteria MK 11 (29,60 cm). Bacterial isolates LB 8 shows bulbs fresh biomass weight (9.83 g) and bulbs fresh biomass shrinkage (7.45) while the bacteria Isolate MK 11 showed the best result of bulb dry weight (2.26 g) and bulbs dry biomass weight (3.41).
机译：从苏拉威西岛上两个不同田间生长的胡葱根际中分离出一些自由生存的根际细菌。将细菌分离物在液体和固体培养基中培养，并进一步测试其产生生物生长素的能力。将L-色氨酸作为生长素的生理学前体添加到培养基中，并使用比色计方法测试IAA的产生。发现六种来自苏拉威西岛的分离株和十个来自南苏拉威西岛的分离株产生生物生长素，而其他40株分离株对IAA产生负面影响。分离物在液体培养基上产生的IAA的量为4.01至8.62 ppm，而在固体培养基中，相同细菌产生的IAA的浓度明显低于液体培养基。因此，培养条件影响细菌分泌IAA。在这16种产生IAA的分离物中，有五种有效的生产者用于植物生长促进测定。分离株MK6-1-1对叶数和鳞茎分ers表现出最好的刺激作用，对分离株LB8进行了研究，以调节更好的鳞茎新鲜生物量，而分离株MK11被发现对鳞茎干重和鳞茎干生物量有最好的影响。 。将液态培养基MK 6-1-1细菌分离物应用到中型青葱种植早期阶段可为青葱的营养生长提供最佳结果，即叶片数（10.75个）和鳞茎分（数（2.75个鳞茎），最好的除外分离细菌MK 11（29,60 cm）显示的植株高度。细菌分离株LB 8显示出鳞茎的新鲜生物量重量（9.83 g）和鳞茎新鲜的生物质收缩量（7.45），而细菌分离物MK 11表现出最佳的鳞茎干重（2.26 g）和鳞茎干生物量重量（3.41）。

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