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首页> 外文期刊>Journal of Molecular Biology Research >ATP16 Genes and Neighboring ORFs Are Duplicated on Chromosome IV in Saccharomyces cerevisiae
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ATP16 Genes and Neighboring ORFs Are Duplicated on Chromosome IV in Saccharomyces cerevisiae

机译：在酿酒酵母中，染色体IV上的ATP16基因和邻近的ORF被复制

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We present evidence that there were two closely linked copies of the ATP16 (YDL004w) gene encoding the delta subunit of F1F0-ATPase complex on chromosome IV in laboratory strains, W303-1A, W303-1B, DC5, LL20, SEY2102, YPH499, and S288C of Saccharomyces cerevisiae. We previously reported that there were 2-3 copies of ATP1 (alpha), ATP2 (beta) and ATP3 (gamma) on chromosomes II, X, and II, respectively. Homologous recombination of the ATP16 with HIS3 (YOR202w), gene walking, and long-PCR analyses showed that two ATP16 were present on the same chromosome as above genes. The gene walking estimated that the two ATP16 were separated by approximately 8.4 kb using ATP16 and its neighboring DNAs as probes, designated the proximal ATP16 to the telomere as ATP16a and to the distal as ATP16b. Although the nucleotide sequences of ATP16a and ATP16b were identical including 833 bases upstream- and 937 bases downstream of ATP16, they might be expressed differently.
机译：我们提供的证据表明，实验室菌株W303-1A，W303-1B，DC5，LL20，SEY2102，YPH499和酿酒酵母的S288C。我们以前曾报告说，在II，X和II号染色体上分别有2-3个ATP1（α），ATP2（β）和ATP3（γ）拷贝。 ATP16与HIS3（YOR202w）的同源重组，基因步移和长PCR分析显示，与上述基因相同，两个ATP16存在于同一条染色体上。基因步移估计使用ATP16及其邻近DNA作为探针将两个ATP16分开约8.4 kb，将端粒的近端ATP16命名为ATP16a，将远端的近端ATP16命名为ATP16b。尽管ATP16a和ATP16b的核苷酸序列相同，包括ATP16上游的833个碱基和下游的937个碱基，但它们的表达可能有所不同。

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