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首页> 外文期刊>Journal of Molecular Biology Research >Expression of Fat and Cholesterol Biomarkers in Meat Goats
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Expression of Fat and Cholesterol Biomarkers in Meat Goats

机译：脂肪和胆固醇生物标志物在肉山羊中的表达

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Consumption of high amounts of saturated fatty acids in meat has been implicated in the onset of cardiovascular disease. Chevon (goat meat) is higher in mono-unsaturated and poly-unsaturated fatty acids than beef and lamb. Limited data is available on the expression of fat and cholesterol biomarkers in meat goats. The objective of this experiment was to determine expression of Acetyl-CoA Carboxylase (ACC1), Apoplipoproteins, A (ApoA1), and B (ApoB) in different breeds of meat goats. Protein sequence alignments were generated to determine conservation for antibody selection. The motif (SMS79 p GL) was conserved in the goat, human, mouse, rat and bovine ACC1 proteins. The ApoA1 and ApoB protein alignments (human, bovine and rabbit) revealed high protein sequence homology. The Enzyme-Linked Immunosorbent Assay (ELISA) was used to determine serum ACC1, ApoA1 and ApoB in Spanish and Myotonic goats. Spanish goats had higher (P<0.05) ACC1 than Myotonic goats. There was a gender effect (P<0.05) where females expressed more ACC1 than males. Breed and gender differences were detected in Spanish and Myotonic goats, with Spanish goats showing 37% more (P<0.05) ApoA1 expression in the blood than Myotonic goats and female goats with 47% higher expression of ApoA1 than males. Inversely, Myotonic goats expressed 35% higher (P<0.05) levels of ApoB than Spanish goats and males had 46% higher (P<0.05) ApoBexpression than females. These data demonstrate that inherent differences exist in lipid metabolism of meat goats and can lead to lipid biomarker assisted breeding programs to produce a heart, healthy red meat for human consumption.
机译：肉中大量饱和脂肪酸的摄入与心血管疾病的发作有关。 Chevon（山羊肉）的单不饱和脂肪酸和多不饱和脂肪酸含量高于牛肉和羊肉。关于肉山羊中脂肪和胆固醇生物标志物表达的数据有限。该实验的目的是确定乙酰辅酶A羧化酶（ACC1），细胞凋亡脂蛋白，A（ApoA1）和B（ApoB）在不同品种的肉山羊中的表达。产生蛋白质序列比对以确定用于抗体选择的保守性。该基序（SMS79 p GL）在山羊，人，小鼠，大鼠和牛ACC1蛋白中均保守。 ApoA1和ApoB蛋白比对（人，牛和兔）显示出高蛋白序列同源性。酶联免疫吸附试验（ELISA）用于测定西班牙山羊和强直性山羊的血清ACC1，ApoA1和ApoB。西班牙山羊的ACC1高于强直性山羊（P <0.05）。有性别效应（P <0.05），其中女性比男性表达更多的ACC1。在西班牙和强直性山羊中发现了品种和性别差异，西班牙山羊血液中的ApoA1表达比强直性山羊和雌性山羊高37％（P <0.05），雌性山羊比男性高47％。相反，强直性山羊的ApoB表达水平比西班牙山羊高35％（P <0.05），雄性山羊的ApoB表达水平比雌性山羊高46％（P <0.05）。这些数据表明，肉山羊的脂质代谢存在固有的差异，并且可以导致脂质生物标志物辅助的育种计划生产出供人类食用的心脏健康的红肉。

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